An ultrasensitive, homogeneous fluorescence quenching immunoassay integrating separation and detection of aflatoxin M1 based on magnetic graphene composites
An ultrasensitive, homogeneous fluorescence quenching immunoassay integrating separation and detection of aflatoxin M1 based on magnetic graphene composites
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基于磁性石墨烯复合材料的黄曲霉毒素M1分离检测超灵敏均相荧光猝灭免疫分析
DOI：
10.1007/s00604-021-04715-2
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发表时间：
2021-01-28
影响因子：
5.7
通讯作者：
Wang，Zhanhui
中科院分区：
文献类型：
--
作者：
Zhang，Xiya;Zhang，Xijie;Wang，Zhanhui
描述了一种同时分离和检测牛奶中黄曲霉毒素m1 （afm1）的均匀荧光猝灭免疫分析法。这种新型检测方法依赖于单克隆抗体（mAb）功能化的fe3o修饰的还原氧化石墨烯（rgo - fe3o -mAb）作为捕获探针和能量受体，结合四甲基罗丹明尸体碱标记的黄曲霉毒素b1 （afb1 -TRCA）作为能量供体。在实验中，afb1 -TRCA在没有afm1的情况下与rGO-Fe 3 O 4 -mAb结合，通过共振能量转移使TRCA的荧光猝灭。值得注意的是，通过使用磁性石墨烯复合材料，免疫分析将样品制备和检测集成为一个步骤，避免了洗涤和离心步骤，并且可以在10分钟内完成分析。优化条件下，afm1的视觉检出限为50 ng L−1，定量检出限为3.8 ng L−1，显著低于相同免疫试剂的荧光偏振法。该方法操作简便，灵敏度高，为afm1的检测提供了有力的工具。图形抽象
A homogeneous fluorescence quenching immunoassay is described for simultaneous separation and detection of aflatoxin M 1 (AFM 1 ) in milk. The novel assay relies on monoclonal antibody (mAb) functionalized Fe 3 O 4 decorated reduced-graphene oxide (rGO-Fe 3 O 4 -mAb) as both capture probe and energy acceptor, combined with tetramethylrhodamine cadaverine-labeled aflatoxin B 1 (AFB 1 -TRCA) as the energy donor. In the assay, AFB 1 -TRCA binds to rGO-Fe 3 O 4 -mAb in the absence of AFM 1 , quenching the fluorescence of TRCA by resonance energy transfer. Significantly, the immunoassay integrates sample preparation and detection into a single step, by using magnetic graphene composites to avoid washing and centrifugation steps, and the assay can be completed within 10 min. Under optimized conditions, the visual and quantitative detection limits of the assay for AFM 1 were 50 and 3.8 ng L −1 , respectively, which were significantly lower than those obtained by fluorescence polarization immunoassay using the same immunoreagents. Owing to its operation and highly sensitivity, the proposed assay provides a powerful tool for the detection of AFM 1 . Graphical abstract