Chemistry of Allenic/Propargylic Anions Generated by Base Treatment of Sulfonylallenes : Synthesis of 1-Alkynyl-1-sulfonylcycloalkanes and Cycloalkanols
Chemistry of Allenic/Propargylic Anions Generated by Base Treatment of Sulfonylallenes : Synthesis of 1-Alkynyl-1-sulfonylcycloalkanes and Cycloalkanols
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磺酰联烯碱处理生成的二烯/丙炔阴离子的化学：1-炔基-1-磺酰基环烷烃和环烷醇的合成
DOI：
10.1016/j.tet.2010.03.071
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发表时间：
2010
期刊：
影响因子：
2.1
通讯作者：
C.
中科院分区：
文献类型：
--
作者：
Kitagaki;S.;Teramoto;S.;Ohta;Y.;Kobayashi;H.;Takabe;M.;Mukai;C.
核孔复合物是大量的多蛋白通道，负责细胞核和细胞质之间的交通，并由大约30种蛋白质组成，称为核孔蛋白（Nup）。我们最近的研究表明，核孔蛋白Rae1和Tpr在维持细胞分裂过程中的纺锤体双极性中起着关键作用。在本研究中，我们发现另一个核孔蛋白Nup88，在有丝分裂过程中与Nup214一起定位在纺锤体上。Nup88的表达与肿瘤的发生发展有关，Nup88已被提议作为肿瘤标志物。Nup88的过表达促进多核细胞的形成。RNAi介导的Nup88的敲低破坏了Nup214的表达和定位，并引起多极纺锤体表型。我们的数据表明Nup88的适当表达对于防止非整倍体形成和肿瘤发生是至关重要的。
Nuclear pore complexes are massive multiprotein channels responsible for traffic between the nucleus and cytoplasm, and are composed of approximately 30 proteins, termed nucleoporins (Nup). Our recent studies indicated that the nucleoporins Rae1 and Tpr play critical roles in maintaining the spindle bipolarity during cell division. In the present study, we found that another nucleoporin, Nup88, was localized on the spindles together with Nup214 during mitosis. Nup88 expression is linked to the progression of carcinogenesis, Nup88 has been proposed as a tumor marker. Overexpression of Nup88 enhanced multinucleated cell formation. RNAi-mediated knockdown of Nup88 disrupted Nup214 expression and localization and caused multipolar spindle phenotypes. Our data indicate that proper expression of Nup88 is critical for preventing aneuploidy formation and tumorigenesis.