[Codon usage bias of Catharanthus roseus].
[Codon usage bias of Catharanthus roseus].
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DOI：
10.4268/cjcmm20162213
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发表时间：
2016-11-01
期刊：
影响因子：
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通讯作者：
Sun, Chao
中科院分区：
文献类型：
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作者：
Li, Ying;Kuang, Xue-Jun;Sun, Chao
本研究旨在通过分析长春花密码子使用偏向性，为异质基因表达、基因预测和物种进化提供指导。分析了长春花30 437个高置信度编码序列的密码子组成和使用偏向，以及大肠杆菌和酿酒酵母稀有密码子在萜类吲哚生物碱生物合成的25个基因中的比例。对C.roseus中的TIA进行了计算。结果表明，该基因的平均GC含量为42.47%；密码子第3个碱基的平均GC含量为35.89%。28个密码子的相对同义密码子使用率(RSCU)大于1，其中26个密码子以A或T结尾。上述25个参与TIA生物合成的基因中，大肠杆菌的稀有密码子远多于酿酒酵母。得出C.roseus主要偏好以A或T结尾的密码子，并遵循以下规则：与酿酒酵母相比，密码子使用与大肠杆菌的差异更大。因此，酿酒酵母可能是更适合异源表达这些基因的宿主。
This study aimed to provide guidance for the heterogenous gene expression, gene prediction and species evolution by analyzing codon usage bias of Catharanthus roseus.The codon composition and usage bias of 30 437 high-confidence coding sequences from C.roseus were analyzed and the proportion of rare codons of Escherichia coli and Saccharomyces cerevisiae in 25 genes involved in the biosynthesis of terpenoid indole alkaloids (TIAs) in C.roseus were calculated.The results showed that the average GC content of the genes was 42.47%; the average GC content of the third bases in codon was 35.89%.The relative synonymous codon usage (RSCU) of 28 codons were greater than 1 and 26 of them ended with A or T.The above 25 genes involved in TIA biosynthesis contained much more rare condons of E.coli than that of S.cerevisiae.It was concluded that C.roseus mainly prefered the codons ending with A or T and the rule of codon usage was more different to E.coli than S.cerevisiae.Thus, S.cerevisiae may be more suitable host for heterologous expression of these genes.