HIGH-DENSITY GENETIC-MAP OF THE BRCA1 REGION OF CHROMOSOME-17Q12-Q21
HIGH-DENSITY GENETIC-MAP OF THE BRCA1 REGION OF CHROMOSOME-17Q12-Q21
复制标题
DOI：
10.1006/geno.1993.1381
复制
发表时间：
1993-09-01
期刊：
影响因子：
4.4
通讯作者：
KING, MC
中科院分区：
文献类型：
--
作者：
ANDERSON, LA;FRIEDMAN, L;KING, MC
为了便于乳腺卵巢癌基因BRCA1的定位克隆，我们构建了染色体17q12-q21上D17S250和GIP之间8.3 cM间隔的高密度遗传图谱。在我们的乳腺癌系列中，标记物被定位在CEPH和扩展激酶中。该图谱包括33个有序多态性，包括12个基因和21个匿名标记，平均每250 kb产生一个多态性。其中25种标记物是基于PCR的系统。多态性基因和标记的顺序为cen-D17S250-D17S518-HER2-THRA1-RARA-D17S80-KRT10-[D17S800-[D17S857]-GAS-D17S856-EDH17B-D17S855-D17S859-D17S858-[PPY-D17S78]-D17S183-EPB3-D17S579-D17S509-[D17S508-D17S190 = D17S810]-D17S791-[D17S181 = D17S806]-D17S797-HOX2B-GP3A-[D17S507 = GIP]-qter. BRCA1位于THRA 1和D17S183之间的区间中间。来自该图谱的标记可用于确定癌症是否与家族中的BRCA 1相关联，以评估肿瘤是否在该区域的基因座处失去杂合性，并鉴定用于表征来自患者和来自肿瘤的染色体重排的探针。
To facilitate the positional cloning of the breast-ovarian cancer gene BRCA1, we constructed a high-density genetic map of the 8.3-cM interval between D17S250 and GIP on chromosome 17q12-q21. Markers were mapped by linkage in tbe CEPH and in extended kindreds in our breast cancer series. The map comprises 33 ordered polymorphisms, including 12 genes and 21 anonymous markers, yielding an average of one polymorphism every 250 kb. Twenty-five of the markers are PCR-based systems. The order of polymorphic genes and markers is cen-D17S250-D17S518-HER2-THRA1-RARA-D17S80-KRT10-[D17S800-[D17S857]-GAS-D17S856-EDH17B-D17S855-D17S859-D17S858-[PPY-D17S78]-D17S183-EPB3-D17S579-D17S509-[D17S508-D17S190 = D17S810]-D17S791-[D17S181 = D17S806]-D17S797-HOX2B-GP3A-[D17S507 = GIP]-qter. BRCA1 lies in the middle of the interval, between THRA1 and D17S183. Markers from this map can be used to determine whether cancer is linked to BRCA1 in families, to evaluate whether tumors have lost heterozygoslty at loci in the region, and to identify probes for characterizing chromosomal rearrangements from patients and from tumors.