Parity between kinetoplast DNA and mini-exon gene sequences supports either clonal evolution or speciation in Trypanosoma rangeli strains isolated from Rhodnius colombiensis, R-pallescens and R-prolixus in Colombia
Parity between kinetoplast DNA and mini-exon gene sequences supports either clonal evolution or speciation in Trypanosoma rangeli strains isolated from Rhodnius colombiensis, R-pallescens and R-prolixus in Colombia
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DOI：
10.1016/s1567-1348(02)00150-8
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发表时间：
2003-05-01
影响因子：
3.2
通讯作者：
Grisard, Edmundo Carlos
中科院分区：
文献类型：
--
作者：
Adolfo Vallejo, Gustavo;Guhl, Felipe;Grisard, Edmundo Carlos
兰氏锥虫是与克氏锥虫有关的动体原虫，在一些拉丁美洲国家得到了广泛的认识和定义，因为这两种锥虫经常出现在锥虫病媒、人类和各种野生和家养哺乳动物的混合感染中。利用两组独立的分子标记，对从自然感染的哥伦比亚红螺旋体、苍白红螺旋体和长尾红螺旋体唾液腺中分离得到的18株朗格丽螺旋体进行了分子鉴定。kDNA和迷你外显子扩增表明两个DNA序列存在二态性：KP1、KP2和KP3或KP2和KP3的产物为kDNA迷你环，而迷你外显子的产物为380或340 bp。在单个菌株中观察到两种关联之一：KP1， KP2和KP3 kDNA产物与340 bp的迷你外显子产物有关，KP2和KP3 kDNA产物与380 bp的迷你外显子产物有关。独立的线粒体和核分子标记表明，与哥伦比亚和其他拉丁美洲国家的特定病媒相关的兰氏疟原虫明显分为两个主要的系统发育群。这些结果支持克隆进化或物种形成，可能是由于它们在三角蝽病媒中寄生条件的二次适应而产生的。(C) 2002 Elsevier Science b.v.版权所有
Trypanosoma rangeli are kinetoplastid protozoa which have been largely recognized and defined in several Latin American countries in relation to T. cruzi, because the two trypanosome species are frequently found in mixed infections in triatominae vectors, humans and a variety of wild and domestic mammals. We report the molecular characterization of 18 T. rangeli strains isolated from the salivary glands of naturally infected Rhodnius colombiensis, R. pallescens and R. prolixus by using two independent set of molecular markers. kDNA and mini-exon amplification indicated dimorphism within both DNA sequences: KP1, KP2 and KP3 or KP2 and KP3 products for kDNA mini-circles and 380 or 340 bp products for the mini-exon. One of two associations was observed within individual strains: KP1, KP2 and KP3 kDNA products with the 340 bp mini-exon product and the KP2 and KP3 kDNA products with the 380 bp mini-exon product. Independent mitochondrial and nuclear molecular markers showed a clear division of T. rangeli into two major phylogenetic groups associated with specific vectors in Colombia and in other Latin America countries. These results support either clonal evolution or speciation in T. rangeli populations, probably derived as a secondary adaptation to their parasitic condition in triatomine vectors. (C) 2002 Elsevier Science B. V. All rights reserved.