Characteristics of internalization of NMDA-type GluRs with antibodies to GluN1 and GluN2B
Characteristics of internalization of NMDA-type GluRs with antibodies to GluN1 and GluN2B
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DOI：
10.1016/j.jneuroim.2020.577427
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发表时间：
2020-12-15
影响因子：
3.3
通讯作者：
Mori, Hisashi
中科院分区：
文献类型：
--
作者：
Takahashi, Yukitoshi;Nishimura, Shigeko;Mori, Hisashi
为了表征NMDA型谷氨酸受体（GluRs）的抗体对NMDA型GluRs的内化，我们制备了针对人GluN 1和GluN 2B的N-末端的兔抗体，并使用共聚焦显微镜检查了HEK 293 T细胞中NMDA型GluRs的内化。NMDA型GluRs的内化发生在与GluN 1和/或GluN 2B抗体孵育后至少10分钟，并且是温度依赖性的。这些研究结果证实，抗体的N-末端的GluN 1和GluN 2B存在于NMDAR脑炎患者的脑脊液中可以介导的NMDA型GluR复合物的迅速内化。
To characterize internalization of NMDA-type glutamate receptors (GluRs) by antibodies to NMDA-type GluRs, we produced rabbit antibodies to N-terminals of human GluN1 and GluN2B, and examined internalization of NMDA-type GluRs in HEK293T cells using confocal microscopy. Internalization of NMDA-type GluRs occurred from at least 10 min after incubation with antibodies to GluN1 and or GluN2B and was temperature-dependent. These findings confirm that antibodies to N-terminals of GluN1 and GluN2B present in the cerebrospinal fluid of patients with NMDAR encephalitis can mediate prompt internalization of NMDA-type GluR complexes.