Single-molecule Taq DNA polymerase dynamics.
Single-molecule Taq DNA polymerase dynamics.
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DOI：
10.1126/sciadv.abl3522
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发表时间：
2022-03-11
期刊：
影响因子：
13.6
通讯作者：
Collins PG
中科院分区：
文献类型：
--
作者：
Turvey MW;Gabriel KN;Lee W;Taulbee JJ;Kim JK;Chen S;Lau CJ;Kattan RE;Pham JT;Majumdar S;Garcia D;Weiss GA;Collins PG
Taq DNA polymerase functions at elevated temperatures with fast conformational dynamics—regimes previously inaccessible to mechanistic, single-molecule studies. Here, single-walled carbon nanotube transistors recorded the motions of Taq molecules processing matched or mismatched template–deoxynucleotide triphosphate pairs from 22° to 85°C. By using four enzyme orientations, the whole-enzyme closures of nucleotide incorporations were distinguished from more rapid, 20-μs closures of Taq’s fingers domain testing complementarity and orientation. On average, one transient closure was observed for every nucleotide binding event; even complementary substrate pairs averaged five transient closures between each catalytic incorporation at 72°C. The rate and duration of the transient closures and the catalytic events had almost no temperature dependence, leaving all of Taq’s temperature sensitivity to its rate-determining open state. Nanocircuit monitoring of Taq activity reveals microsecond single-molecule dynamics up to 85°C.
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影响因子：
4.3
作者：
Miller BR 3rd;Parish CA;Wu EY
通讯作者：
Wu EY
DOI：
10.1073/pnas.85.24.9436
发表时间：
1988-12-01
影响因子：
11.1
作者：
INNIS, MA;MYAMBO, KB;BROW, MAD
通讯作者：
BROW, MAD
影响因子：
15
作者：
An, L;Owens, JM;Liu, J
通讯作者：
Liu, J
影响因子：
8
作者：
Diaz, Juan E.;Lin, Chun-Shi;Weiss, Gregory A.
通讯作者：
Weiss, Gregory A.
通过高分辨率的单分子酶学观察溶菌酶的闭合和打开动作。
影响因子：
4
作者：
Akhterov MV;Choi Y;Olsen TJ;Sims PC;Iftikhar M;Gul OT;Corso BL;Weiss GA;Collins PG
通讯作者：
Collins PG