Somatic embryogenesis and direct as well as indirect organogenesis in Lilium pumilum DC. Fisch., an endangered ornamental and medicinal plant
Somatic embryogenesis and direct as well as indirect organogenesis in Lilium pumilum DC. Fisch., an endangered ornamental and medicinal plant
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百合 DC 的体细胞胚胎发生以及直接和间接器官发生。
DOI：
10.1080/09168451.2016.1194178
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发表时间：
2016-01-01
影响因子：
1.6
通讯作者：
Sun, HongMei
中科院分区：
文献类型：
--
作者：
Zhang, Jing;Gai, MeiZhu;Sun, HongMei
通过毒莠定、α -萘乙酸（NAA）和6 -苄基腺嘌呤（BA）成功调控了细叶百合的体细胞胚胎发生和器官发生。在器官发生中，在含有2.0mg/L BA和0.2mg/L NAA的Murashige和Skoog（MS）培养基上，从鳞片直接获得的芽再生频率最高（92.5%），而在添加1.0mg/L BA和0.5mg/L NAA的MS培养基上，叶片形成了器官发生愈伤组织（OC）。继代培养后，76.7%的OC再生出芽。在体细胞胚胎发生中，毒莠定和NAA的组合增加了胚性愈伤组织（EC）的数量，在90.7%的外植体上达到最大值，每个外植体形成11个体细胞胚胎（SE）。很容易观察到EC和OC在细胞形态和细胞分化命运上的差异。SE最初通过外源或内源途径形成。心形SE中原表皮的出现以及椭圆形SE中两极的芽 - 根发育表明了真正的体细胞胚胎发生。该方案为细叶百合的再生模式提供了一种新的详细调控和组织学检测方法。
Somatic embryogenesis and organogenesis in Lilium pumilum were successfully regulated by picloram, -naphthaleneacetic acid (NAA), and 6-benzyladenine (BA). In organogenesis, the highest shoot regeneration frequency (92.5%) was obtained directly from bulb scales on Murashige and Skoog (MS) medium containing 2.0mgL(-1) BA and 0.2mgL(-1) NAA, while organogenic callus (OC) formed from leaves on MS medium supplemented with 1.0mgL(-1) BA and 0.5mgL(-1) NAA. Following subculture, 76.7% of OC regenerated shoots. In somatic embryogenesis, the combination of picloram and NAA increased the amount of embryogenic callus (EC) that formed with a maximum on 90.7% of all explants which formed 11 somatic embryos (SEs) per explant. Differences between EC and OC in cellular morphology and cell differentiation fate were easily observed. SEs initially formed via an exogenous or an endogenous origin. The appearance of a protoderm in heart-shaped SE and the bipolar shoot-root development in oval-shaped SE indicated true somatic embryogenesis. This protocol provides a new and detailed regulation and histological examination of regeneration pattern in L. pumilum.