Genome-wide identification and analysis of the WUSCHEL-related homeobox (WOX) gene family in allotetraploid Brassica napus reveals changes in WOX genes during polyploidization
Genome-wide identification and analysis of the WUSCHEL-related homeobox (WOX) gene family in allotetraploid Brassica napus reveals changes in WOX genes during polyploidization
复制标题
对异源四倍体甘蓝型油菜 WUSCHEL 相关同源盒 (WOX) 基因家族的全基因组鉴定和分析揭示了多倍化过程中 WOX 基因的变化
DOI：
10.1186/s12864-019-5684-3
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发表时间：
2019-04-25
期刊：
影响因子：
4.4
通讯作者：
Wang, Jianbo
中科院分区：
文献类型：
--
作者：
Li, Mengdi;Wang, Ruihua;Wang, Jianbo
背景：WUSCHEL相关同源盒（WOX）基因编码植物特异性同源盒（HB）转录因子，在植物的生长发育中起重要作用。迄今为止，WOX基因已在许多多倍体植物（如棉花和烟草）中被鉴定和分析，但在多倍体化过程中的进化分析却很少。随着基因组测序的完成，异源四倍体甘蓝型油菜及其二倍体祖先（B. rapa和B.结果：在异源四倍体B中分别鉴定出52、25和29个WOX基因。napus（2n = 4x = 38，AnCn），Angenome供体B. rapa（2n = 2x = 20，Ar）和Cn基因组供体B。oleracea（2n = 2x = 18，Co.）在B中所有鉴定的WOX基因。将甘蓝型油菜及其二倍体后代分为3个进化枝，并选择这些基因进行基因结构和染色体定位分析。结果表明，至少有70%和67%的WOX基因分别保持相同的基因结构和染色体上的相对位置，表明WOX基因在B.在多倍体化过程中，油菜在DNA水平上高度保守。此外，对WOX基因附近的重复基因和转座元件（TE）的分析表明，全基因组三倍体（WGT）事件、片段重复和丰富的TE在B中WOX基因家族的扩展中起重要作用。油菜。此外，WOX基因对的表达谱与进化关系的分析表明，WOX基因家族在多倍化过程中可能在转录调控水平发生了变化。结论：本研究结果增加了我们对B中WOX基因的了解。甘蓝型油菜及其二倍体亲本的WOX基因的克隆和鉴定，为进一步研究这些品种的WOX基因提供了丰富的资源。此外，从基因数量、基因结构、基因相对位置和基因表达等方面探讨了WOX基因在多倍体化过程中的变化，为今后的多倍体化分析提供参考。
Background:WUSCHEL-related homeobox (WOX) genes encoding plant-specific homeobox (HB) transcription factors play important roles in the growth and development of plants. To date, WOX genes has been identified and analyzed in many polyploids (such as cotton and tobacco), but the evolutionary analysis of them during polyploidization is rare. With the completion of genome sequencing, allotetraploid Brassica napus and its diploid progenitors (B. rapa and B. oleracea) are a good system for studying this question.Results:In this study, 52, 25 and 29 WOX genes were identified in allotetraploid B. napus (2n = 4x = 38, AnCn), the Angenome donor B. rapa (2n = 2x = 20, Ar) and the Cngenome donor B. oleracea (2n = 2x = 18, Co), respectively. All identified WOX genes in B. napus and its diploid progenitors were divided into three clades, and these genes were selected to perform gene structure and chromosome location analysis. The results showed that at least 70 and 67% of WOX genes maintained the same gene structure and relative position on chromosomes, respectively, indicating that WOX genes in B. napus were highly conserved at the DNA level during polyploidization. In addition, the analysis of duplicated genes and transposable elements (TEs) near WOX genes showed that whole-genome triplication (WGT) events, segmental duplication and abundant TEs played important roles in the expansion of the WOX gene family in B. napus. Moreover, the analysis of the expression profiles of WOX gene pairs with evolutionary relationships suggested that the WOX gene family may have changed at the transcriptional regulation level during polyploidization.Conclusions:The results of this study increased our understanding of the WOX genes in B. napus and its diploid progenitors, providing a rich resource for further study of WOX genes in these species. In addition, the changes in WOX genes during the process of polyploidization were discussed from the aspects of gene number, gene structure, gene relative location and gene expression, which provides a reference for future polyploidization analysis.