The use of PAMAM dendrimers in the efficient transfer of genetic material into cells.
The use of PAMAM dendrimers in the efficient transfer of genetic material into cells.
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DOI：
10.1016/s1461-5347(00)00273-x
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发表时间：
2000-07-01
期刊：
影响因子：
--
通讯作者：
Baker Jr
中科院分区：
文献类型：
--
作者：
Eichman;Bielinska;Baker Jr
过去十年中，聚酰胺胺 (PAMAM) 树枝状聚合物在从材料科学到生物医学的各个学科中稳步普及。这部分归因于它们在从计算机碳粉到医疗诊断等各种应用中的使用。 PAMAM 树枝状聚合物安全且无免疫原性，可作为高效阳离子聚合物载体，将遗传物质传递到细胞中。对于体外基因转移，它们已被证明比阳离子脂质体或其他阳离子聚合物（例如聚乙烯亚胺、聚赖氨酸）更有效或更有效。本文将重点介绍 PAMAM 树枝状聚合物作为非病毒基因传递载体的应用，从最初发现这种能力到最新的实验发现。
Polyamidoamine (PAMAM) dendrimers have steadily grown in popularity in the past decade in a variety of disciplines, ranging from materials science to biomedicine. This can be attributed in part to their use in applications that range from computer toners to medical diagnostics. PAMAM dendrimers are safe and nonimmunogenic, and can function as highly efficient cationic polymer vectors for delivering genetic material into cells. They have been shown to be as efficient or more efficient than either cationic liposomes or other cationic polymers (e.g. polyethylenimine, polylysine) for in vitro gene transfer. This article will focus on the application of PAMAM dendrimers as a nonviral gene delivery vector from the initial discovery of this capacity to the most recent experimental findings.