Screening of kinase substrates using kinase knockout mutants.
Screening of kinase substrates using kinase knockout mutants.
复制标题
DOI：
10.1007/978-1-4939-2648-0_4
复制
发表时间：
2015
影响因子：
--
通讯作者：
T. Umezawa
中科院分区：
文献类型：
--
作者：
T. Umezawa
蛋白激酶被广泛认为是各种信号传导过程的主要调节剂，特别是在包括植物在内的真核生物中。为了了解它们在信号转导途径中的作用，有必要确定哪些蛋白质被这些酶磷酸化。最近的研究已经应用比较磷酸化蛋白质组学方法来鉴定植物中的蛋白激酶底物。结果表明，激酶敲除突变体可用于通过这种比较分析筛选蛋白激酶底物。本文介绍了利用激酶敲除突变体进行实验设计和比较分析的一些技术要点。
Protein kinases are widely known to be major regulators of various signaling processes, particularly in eukaryotes, including plants. To understand their role in signal transduction pathways, it is necessary to determine which proteins are phosphorylated by these enzymes. Recent studies have applied a comparative phosphoproteomic approach to identify protein kinase substrates in plants. The results demonstrated that kinase knockout mutants are useful for screening protein kinase substrates via such a comparative analysis. Here some technical points are described for the experimental design and comparative analysis using kinase knockout mutants.