A Comparison Study on the Degradation and Cytocompatibility of Mg-4Zn-xSr Alloys in Direct Culture
A Comparison Study on the Degradation and Cytocompatibility of Mg-4Zn-xSr Alloys in Direct Culture
复制标题
直接培养中Mg-4Zn-xSr合金降解及细胞相容性的比较研究
DOI：
10.1021/acsbiomaterials.6b00684
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发表时间：
2017-04-01
影响因子：
5.8
通讯作者：
Liu, Huinan
中科院分区：
文献类型：
--
作者：
Cipriano, Aaron F.;Sallee, Amy;Liu, Huinan
本文报道了骨髓间充质干细胞（BMSCs）在4种Mg-4 Zn-xSr合金中的直接培养行为（x = 0.15、0.5、1.0、1.5重量%），分别命名为A1 r41 A、B、C和D;系统地比较了不同细胞类型直接培养条件下Cr 141合金的降解情况及其生物学效应，各自的媒体。直接培养法，其中细胞直接接种到样品的表面上，被用来研究在细胞-生物材料界面的细胞反应在体外。结果表明，BMSCs粘附并保持在所有Al 2 O3 - 41合金的表面上的活力，但降解较快的Al 2 O3 - 41 A和Al 2 O3 - 41 B合金显示粘附于其表面的活BMSCs的量显著较低。此外，BMSCs粘附到培养板周围的样品不受溶解的降解产物从Al 2 O3 - 41合金。比较研究的结果表明，镁基生物材料在不同培养体系中的体外降解速率可能主要受培养基缓冲能力的影响（即，HCO 3-浓度），并且在较小程度上，（D）-葡萄糖浓度。比较研究还表明，BMSCs比H9人胚胎干细胞和人脐静脉内皮细胞更稳健地筛选镁基生物材料的细胞相容性。通常，BMSC在细胞-材料界面处的粘附性和活力与合金降解速率成反比。本研究提供了一种临床相关的体外培养系统，用于在直接培养中筛选生物可吸收合金，并为确定镁基生物材料的降解速率提供了有价值的指导。
This article reports the behaviors of bone marrow -derived mesenchymal stem cells (BMSCs) in the direct culture with four Mg-4Zn-xSr alloys (x = 0.15, 0.5, 1.0, 1.5 wt %), designated as ZSr41A, B, C, and D, respectively; and a systematic comparison on the degradation of the ZSr41 alloys and their biological impact in the direct culture with different cell types in their respective media. The direct culture method, in which cells are seeded directly onto the surface of the sample, was used to investigate cellular responses at the cell-biomaterial interface in vitro. The results showed that BMSCs adhered and remained viable on the surfaces of all ZSr41 alloys, but the faster degrading ZSr41A and ZSr41B alloys showed a significantly lower amount of viable BMSCs adhered to their surfaces. Moreover, BMSCs adhered to the culture plate surrounding the samples were unaffected by the solubilized degradation products from the ZSr41 alloys. The results from the comparison study showed that the in vitro degradation rates of Mg-based biomaterials in different culture systems might be mostly affected by media buffer capacity (i.e., HCO3- concentration), and to a lesser extent, (D)-glucose concentration. The comparison study also indicated that BMSCs were more robust than H9 human embryonic stem cells and human umbilical vein endothelial cells for screening the cytocompatibility of Mg-based biomaterials. In general, the adhesion and viability of BMSCs at the cell -material interface were inversely proportional to the alloy degradation rates. This study presented a clinically relevant in vitro culture system for screening bioresorbable alloys in direct culture, and provided valuable guidelines for determining the degradation rates of Mg-based biomaterials.