Monovalent cation composition and ATP and lipid content of irreversibly sickled cells.
Monovalent cation composition and ATP and lipid content of irreversibly sickled cells.
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不可逆镰状细胞的单价阳离子组成以及 ATP 和脂质含量。
DOI：
10.1182/blood.v51.6.1169.bloodjournal5161169
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发表时间：
1978
期刊：
影响因子：
20.3
通讯作者：
S. Shohet
中科院分区：
文献类型：
--
作者：
M. Clark;R. Unger;S. Shohet
使用不连续的 Stractan 梯度将镰状细胞的异质群体分离成包含不同比例的网织红细胞、成熟盘状细胞和不可逆镰状细胞 (ISC) 的离散亚群。这些制剂均质性的改善以及 ISC 产量的提高，使我们能够区分细胞成熟的影响和不可逆镰状化的影响。通过这些细胞制剂，我们开始定义 ISC 的物理特性。我们证实了天然 ISC 的阳离子组成存在明显异常。 ISC 中 ATP 的测量并未证实先前关于 ATP 缺乏的报告。最后，没有证据表明 ISC 形成过程中膜脂大量损失。
Discontinuous Stractan gradients were used to separate heterogeneous populations of sickle cells into discrete subpopulations containing varying proportions of reticulocytes, mature discoid cells, and irreversibly sickled cells (ISC). The improved homogeneity of these preparations, together with an enhanced yield of ISC, allowed us to distinguish effects of cell maturation from those of irreversible sickling. With these cell preparations we have begun to define physical properties of ISC. We confirmed the marked abnormalities in cation composition of native ISC. Measurements of ATP in ISC did not substantiate prior reports of ATP deficits. Finally, no evidence for substantial loss of membrane lipids during the process of ISC formation could be demonstrated.