Bactericidal Effect of a Novel Alkaline EDTA Root Canal Cleaning Solution.
Bactericidal Effect of a Novel Alkaline EDTA Root Canal Cleaning Solution.
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DOI：
10.1055/s-0041-1723067
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发表时间：
2021-07
影响因子：
--
通讯作者：
Handa K
中科院分区：
文献类型：
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作者：
Sato T;Fujimaki R;Suzuki J;Hamada N;Tani-Ishii N;Handa K
目的评价乙二胺四乙酸根管冲洗液的杀菌效果和细胞毒性。该冲洗液能有效去除根管内有机物和玷污层。 我们制备了一种强碱性EDTA(AE)溶液，其酸缓冲能力类似于次氯酸钠。材料与方法采用1%、2%、3%浓度的 -AE。通过测定菌落数和生物被膜清除率来评价AE对粪肠球菌的杀菌效果。使用一种Live/Dead BacLight细菌生存能力试剂盒对生物膜进行可视化。用CCK-8细胞计数法检测经AE处理的细胞的存活率。组间比较采用统计分析、 单因素方差分析和邓尼特多重比较检验。结果当AE浓度为3%和2%时， 的细胞存活率和生物膜形成能力明显下降。AE具有浓度依赖性的杀菌作用。未观察到任何浓度的AE对正常人成纤维细胞的损伤。结论 结果表明，AE液可作为一种有效的根管冲洗剂去除细菌生物被膜。
Objectives In this study, we aimed to evaluate the bactericidal effect and cytotoxicity of an ethylenediaminetetra-acetic acid (EDTA)-based root canal irrigant solution capable of efficiently removing both the organic matter and the smear layer. We prepared a strong alkaline EDTA (AE) solution with an acid buffer capacity similar to that of sodium hypochlorite. Materials and Methods AE was used at concentrations of 1%, 2%, and 3%. The bactericidal effect of AE on Enterococcus faecalis was evaluated by determining the colony number and biofilm removal rate. Biofilms were visualized using a Live/Dead BacLight bacterial viability kit. Viability of AE-treated cells were determined using a CCK-8 cell counting assay. Statistical Analysis One-way analysis of variance followed by a Dunnett’s multiple comparison test were used for comparisons among groups. Results Significant reduction in cell viability and biofilm formation were observed in case of 3% and 2% AE. AE exerted bactericidal effects in a concentration-dependent manner. Damage of normal human fibroblasts was not observed at any of the AE concentrations. Conclusions Our results suggest that the AE solution could be used as an effective canal irrigant for the removal of bacterial biofilm.