Genetic interactions between protein phosphatases CaPtc2p and CaPph3p in response to genotoxins and rapamycin in Candida albicans
Genetic interactions between protein phosphatases CaPtc2p and CaPph3p in response to genotoxins and rapamycin in Candida albicans
复制标题
白色念珠菌中蛋白磷酸酶 CaPtc2p 和 CaPph3p 响应基因毒素和雷帕霉素的遗传相互作用
DOI：
10.1111/1567-1364.12012
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发表时间：
2013-01-01
影响因子：
3.2
通讯作者：
Jiang, Linghuo
中科院分区：
文献类型：
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作者：
Feng, Jinrong;Zhao, Yunying;Jiang, Linghuo
在酿酒酵母细胞中，两种 PP2C 蛋白磷酸酶 ScPtc2p 和 ScPtc3p 以及 PP4 蛋白磷酸酶 ScPph3 负责 DNA 甲基化剂甲基磺酸甲酯 (MMS) 诱导的 DNA 损伤后 ScRad53p 去磷酸化。在这项研究中，我们表明，在 DNA 损伤恢复过程中，CaPtc2p 不是 CaRad53p 去磷酸化所必需的，而白色念珠菌中的 CaPph3p 也是如此。然而，CaPPH3 的缺失对缺乏 CaPTC2 的白色念珠菌细胞对 MMS 和 DNA 合成抑制剂羟基脲 (HU) 的敏感性具有累加效应。此外，CaPPH3 的缺失促进白色念珠菌细胞的体外丝状化。此外，在白色念珠菌细胞对雷帕霉素的敏感性中，CaPTC2的突变与CaPPH3的突变是上位的。因此，CaPtc2p 和 CaPph3p 可能在白色念珠菌细胞中雷帕霉素 (TOR) 信号传导的靶标中发挥作用。
In Saccharomyces cerevisiae cells, both of the two PP2C protein phosphatases ScPtc2p and ScPtc3p and the PP4 protein phosphatase ScPph3 are responsible for ScRad53p dephosphorylation after the DNA methylation agent methylmethane sulfonate (MMS)-induced DNA damage. In this study, we show that CaPtc2p is not required for the CaRad53p dephosphorylation during the recovery from DNA damage, as is CaPph3p in Candida albicans. However, deletion of CaPPH3 has an additive effect on the sensitivity of C. albicans cells lacking CaPTC2 to MMS and the DNA synthesis inhibitor hydroxyurea (HU). In addition, deletion of CaPPH3 promotes in vitro filamentation of C. albicans cells. Furthermore, mutation of CaPTC2 is epistatic to that of CaPPH3 in the sensitivity of C. albicans cells to rapamycin. Therefore, CaPtc2p and CaPph3p might play a role in the target of rapamycin (TOR) signaling in C. albicans cells.