A genome-wide analysis of immune responses in Drosophila
A genome-wide analysis of immune responses in Drosophila
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DOI：
10.1073/pnas.261573998
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发表时间：
2001-12-18
影响因子：
11.1
通讯作者：
Hetru, C
中科院分区：
文献类型：
--
作者：
Irving, P;Troxler, L;Hetru, C
寡核苷酸DNA微阵列用于全基因组分析免疫挑战感染革兰氏阳性或革兰氏阴性细菌，或真菌的果蝇。除了一组已建立的免疫防御基因的表达，一个以前看不见的免疫诱导基因的显着数量被发现。特别感兴趣的基因包括corin和Stubble样基因，这两个都有一个II型跨膜结构域;复活节和蛇样基因，这可能履行复活节和蛇在Toll通路的作用;和一个伪装样基因，可能参与酶的调节。微阵列数据还有助于大大减少大型基因组中靶基因的数量，如蛋白酶，有助于指导未来突变研究的选择。许多上调的基因符合当前宿主防御的概念框架，而另一些上调的基因包括大量功能未知的基因，为研究提供了新的途径。
Oligonucleotide DNA microarrays were used for a genome-wide analysis of immune-challenged Drosophila infected with Grampositive or Gram-negative bacteria, or with fungi. Aside from the expression of an established set of immune defense genes, a significant number of previously unseen immune-induced genes were found. Genes of particular interest include corin- and Stubble-like genes, both of which have a type II transmembrane domain; easter- and snake-like genes, which may fulfil the roles of easter and snake in the Toll pathway; and a masquerade-like gene, potentially involved in enzyme regulation. The microarray data has also helped to greatly reduce the number of target genes in large gene groups, such as the proteases, helping to direct the choices for future mutant studies. Many of the up-regulated genes fit into the current conceptual framework of host defense, whereas others' including the substantial number of genes with unknown functions, offer new avenues for research.