Prostate epithelial cell lines form spheroids with evidence of glandular differentiation in three-dimensional Matrigel cultures
Prostate epithelial cell lines form spheroids with evidence of glandular differentiation in three-dimensional Matrigel cultures
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DOI：
10.1054/bjoc.2001.1967
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发表时间：
2001-08-17
影响因子：
8.8
通讯作者：
Maitland, NJ
中科院分区：
文献类型：
--
作者：
Lang, SH;Sharrard, RM;Maitland, NJ
正常（PNT 2-C2）和转移性（PC-3）前列腺细胞系在Matrigel中生长，以观察与单层培养相比对形态和表型的影响。在单层培养中，PNT 2-C2显示出典型的圆形/立方形上皮形态，具有紧密的细胞缔合，而在Matrigel中，它们形成光滑的球状体，紧密地挤满了细胞。在单层和Matrigel中，PNT 2-C2具有分化的管腔上皮表型，具有高表达的细胞角蛋白8、前列腺特异性抗原（PSA）、前列腺特异性膜抗原（PSMA）、E-钙粘蛋白和桥粒芯糖蛋白。与此相反，PC-3细胞具有上皮/间充质形态的单层松散的细胞与细胞接触和伪足的延伸。免疫组化表型表明细胞未分化，表达高水平的波形蛋白，β 1整合素，CD 44和低表达的细胞角蛋白8。在Matrigel中，它们形成光滑和不规则的球体，其具有由单个细胞层包围的管腔。Matrigel还影响PSA、PSMA和CD 44的表达。这些结果表明，基质胶培养物可以诱导最初具有基础表型的前列腺癌细胞的形态分化。(C)2001年癌症研究运动http://www.bicancer.com。
Normal (PNT2-C2) and metastatic (PC-3) prostate cell lines were grown in Matrigel to observe the effects on morphology and phenotype in comparison to monolayer culture. In monolayer cultures, PNT2-C2 showed typical round/cuboidal epithelial morphology, with tight cell associations, whereas in Matrigel they formed smooth spheroids, tightly packed with cells. In both monolayer and Matrigel, PNT2-C2 had a differentiated luminal epithelial phenotype with high expression of cytokeratin 8, prostate specific antigen (PSA), prostate specific membrane antigen (PSMA), E-cadherin and desmoglein. In contrast, PC-3 cells possessed an epithelial/mesenchyme morphology in monolayer with loose cell to cell contact and pseudopodial extensions. Immunohistochemical phenotyping indicated the cells were undifferentiated, expressing high levels of vimentin, beta1 integrin, CD44 and low expression of cytokeratin 8. In Matrigel they formed smooth and irregular spheroids, which had a lumen surrounded by a single cell layer. Matrigel also influenced the expression of PSA, PSMA and CD44. These results indicate that Matrigel culture can induce morphological differentiation of prostate cancer cells which initially had a basal phenotype. (C) 2001 Cancer Research Campaign http://www.bicancer.com.