Reciprocal and nonreciprocal recombination in diploid clones from Bacillus subtilis protoplast fusion: Association with the replication origin and terminus.
Reciprocal and nonreciprocal recombination in diploid clones from Bacillus subtilis protoplast fusion: Association with the replication origin and terminus.
复制标题
枯草芽孢杆菌原生质体融合二倍体克隆中的相互和非相互重组：与复制起点和终点的关联。
DOI：
10.1073/pnas.80.5.1426
复制
发表时间：
1983
影响因子：
11.1
通讯作者：
Rollin D. Hotchkiss
中科院分区：
文献类型：
--
作者：
M. Gabor;Rollin D. Hotchkiss
枯草芽孢杆菌融合后再生的主要异源二倍体细菌，虽然通常是非互补二倍体，但在谱系分析中表现为多能系统。产生完整的相互重组（RR）后代的个体二倍体菌落（通常伴随一个或两个亲本）占总重组形成单位的10-30%。RR（8-11个基因的倒数）通常在单个菌落之间和菌落内以相等的数量出现。新的细菌，他们证明，整个父母的基因组在一个二倍体原生质体中被带到一起，保留为两个独立的复制子，能够进行经典的真核配子发生的重组特征。亲本或重组基因组也经历多轮重组，而没有专性分离，并且通常不是相互的。二倍体重组克隆，共享链霉素抗性，但互惠的营养缺陷型标记，已显示出部分能力，使一个兼性转变的染色体表达。它们还产生了两种类型的原养型：一种稳定的（可能是单倍体和重组体）和一种不稳定的（二倍体和以低频率暂时互补）。由此可见，染色体消失可能影响亲本染色体和重组染色体，但不干扰重组。在所有重组体和那些从单一的二倍体克隆的数量和染色体分布的交叉分析表明，在复制起点和末端的区域，可能是这些网站与细胞壁或膜的协会的结果增加的频率交换。
The primary heterodiploid bacteria regenerated after Bacillus subtilis fusion, although generally noncomplementing diploids, behave in pedigree analysis as multipotential systems. Individual diploid colonies yielding complete reciprocal recombinant (RR) progeny-often accompanied by one or both parents-constitute 10-30% of the total recombinant-forming units. The RR (reciprocal for 8-11 genes) usually occur in equivalent numbers both among and within individual colonies. Novel for bacteria, they demonstrate that entire parental genomes brought together within a diploid protoplast are retained as two independent replicons able to undergo classical recombination characteristic of eukaryotic gametogenesis. Parental or recombinant genomes are also subject to multiple rounds of recombination without obligate segregation and often not reciprocal. Diploid recombinant clones, sharing streptomycin resistance but reciprocal for auxotrophic markers, have displayed a partial ability to make a facultative shift in chromosome expression. They have also produced two types of prototrophs: a stable one (presumably haploid and recombinant) and an unstable one, (diploid and temporarily complementing at low frequency). It follows that chromosome extinction may affect both parental and recombinant chromosomes and does not interfere with recombination. Analysis of the number and chromosomal distribution of crossovers in all recombinants and those from single diploid clones shows increased frequency of exchange in the regions of the replication origin and terminus, possibly a result of the association of these sites with the cell wall or membrane.