Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins
Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins
复制标题
羧基修饰磁性颗粒和水两相萃取的整合用于选择性分离蛋白质。
DOI：
10.1016/j.talanta.2011.03.055
复制
发表时间：
2011-07-15
期刊：
影响因子：
6.1
通讯作者：
Zhang, Yukui
中科院分区：
文献类型：
--
作者：
Gai, Qingqing;Qu, Feng;Zhang, Yukui
磁性颗粒吸附法和双水相萃取法都是一种简单、快速、低成本的蛋白质分离方法。以蛋白质的等电点、溶液pH值和离子强度为指标，研究了羧基修饰磁性粒子对蛋白质的选择性吸附。PEG/硫酸盐双水相体系在磁吸附前后对蛋白质具有选择性分离和萃取作用。本文讨论并比较了磁吸附-ATPE和ATPE-磁吸附两种联用方法对溶菌酶、牛血清白蛋白、胰蛋白酶、细胞色素C和肌球蛋白混合蛋白的分离效果。将磁吸附-ATPE联用的方法应用于人血清的分离。(C)2011 Elsevier B. V.保留所有权利。
Both of the magnetic particle adsorption and aqueous two-phase extraction (ATPE) were simple, fast and low-cost method for protein separation. Selective proteins adsorption by carboxyl modified magnetic particles was investigated according to protein isoelectric point, solution pH and ionic strength. Aqueous two-phase system of PEG/sulphate exhibited selective separation and extraction for proteins before and after magnetic adsorption. The two combination ways, magnetic adsorption followed by ATPE and ATPE followed by magnetic adsorption, for the separation of proteins mixture of lysozyme, bovine serum albumin, trypsin, cytochrome C and myloglobin were discussed and compared. The way of magnetic adsorption followed by ATPE was also applied to human serum separation. (C) 2011 Elsevier B.V. All rights reserved.