Female-to-male sex reversal in orange-spotted grouper (Epinephelus coioides) caused by overexpressing of Amh in vivo
Female-to-male sex reversal in orange-spotted grouper (Epinephelus coioides) caused by overexpressing of Amh in vivo
复制标题
体内 Amh 过度表达导致橙斑石斑鱼 (Epinephelus coioides) 雌雄逆转
DOI：
10.1093/biolre/ioy157
复制
发表时间：
2018-12-01
影响因子：
3.6
通讯作者：
Lin, Haoran
中科院分区：
文献类型：
--
作者：
Han, Yulong;Peng, Cheng;Lin, Haoran
脊椎动物的性别决定涉及多种机制。斜带石斑鱼是一种先雌后雄的硬骨鱼，是研究性命运调控的理想模型。在这里，我们报告的女性到男性的性逆转在少年橙斑石斑鱼引起的过度表达抗苗勒氏管激素（AMH）。组织分布分析表明，amh和amhrII主要在性腺中表达，且在睾丸中的表达量明显高于卵巢。在性腺中，amh的表达定位于睾丸精原细胞周围的支持细胞和成熟卵巢的透明细胞，amhr Ⅱ的表达定位于睾丸的支持细胞和卵巢的卵母细胞。在质粒喂养实验中，观察到雌性相关基因和血清17 β-雌二醇水平降低，雄性相关基因和血清11-酮睾酮水平升高，卵巢退化和精原细胞增殖。这些结果表明，AMH过表达质粒喂养可以诱导石斑鱼的雌性向雄性转变。
A variety of mechanisms are involved in sex determination in vertebrates. The orange-spotted grouper (Epinephelus coioides), a teleost fish, functions first as females and later as a male and is an ideal model to investigate the regulation of sexual fate. Here, we report female-to-male sex reversal in juvenile orange-spotted groupers caused by overexpressing anti-Mullerian hormone (Amh). Tissue distribution analyses showed that amh and amhrII primarily expressed in the gonad, and expression level in the testis wasmuch higher than that in the ovary. In gonads, the expression of amh was located in the Sertoli cells around spermatogonia of the testis and in the zona pellucida of the mature ovary, and the expression of amhrII was located in the Sertoli cells of the testis and in the oocytes of the ovary. Decrease in female-related genes and serum 17 beta-estradiol level, increase in male-related genes and serum 11-ketotestosterone, ovarian regression, and spermatogonia proliferation were observed during plasmid feeding experiment. These results illustrate that amh overexpression plasmid feeding can induce a female-to-male transition in grouper.