Influence of Osmolality and Acidity on Fertilized Eggs and Larvae of Olive Flounder (Paralichthys olivaceus).
Influence of Osmolality and Acidity on Fertilized Eggs and Larvae of Olive Flounder (Paralichthys olivaceus).
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DOI：
10.12717/dr.2020.24.1.19
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发表时间：
2020-03-01
期刊：
影响因子：
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通讯作者：
Yeo, In-Kyu
中科院分区：
文献类型：
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作者：
Kim, Ki-Hyuk;Moon, Hye-Na;Yeo, In-Kyu
水的pH值是对海洋和淡水生物施加选择压力的主要环境因子之一。在这里，我们关注pH值对生物体维持体内平衡能力的影响，并研究酸化对橄榄比目鱼（palichthys olivaceus）早期发育过程中免疫相关基因和渗透压的影响。我们的研究目的是确定不同的pH值水平对橄榄虾受精卵和幼虫的影响。人工引入橄榄虾配子，受精卵分别在pH 4.0（低）、6.0和8.0（相当于天然海水；对照）下孵育。我们发现，在pH值为4.0时，所有的鸡蛋都从水柱中下沉。38 h后，这些卵发育缓慢。在pH 4.0和6.0时，孵化速度较慢，在pH 4.0时根本没有孵化。基因表达结果显示，除双细胞期外，半胱天冬酶和半乳糖凝集素-1在囊胚至孵化前期均有表达。hsp70在所有pH水平下均稳定表达5天。受精卵的渗透压在每个阶段和pH值水平上略有不同。由此可见，低pH对橄榄虾受精卵是不利的。
The pH of water is one of the main environmental factors exerting selective pressure on marine and freshwater organisms. Here, we focus on the influence of pH on an organism's ability to maintain homeostasis and investigate the effects of acidification on immunity-related genes and osmotic pressure during early development of the olive flounder, Paralichthys olivaceus. The aim of our study was to determine the influence of various pH levels on the fertilized eggs and larvae of P. olivaceus. Gametes of P. olivaceus were artificially introduced and the resulting fertilized eggs were incubated at pH 4.0 (low), 6.0, and 8.0 (equivalent to natural sea water; control). We found that all eggs sank from the water column at pH 4.0. After 38 h, these eggs showed slow development. Hatching occurred more slowly at pH 4.0 and 6.0 and did not occur at all at pH 4.0. Result of gene expression, caspase and galectin-1 were expressed from the blastula to pre-hatch stages, with the exception of the two-cell stage. HSP 70 was also steadily expressed at all pH levels over the five days. The osmolality of fertilized eggs differed marginally at each stage and across pH levels. So, this results demonstrates that low pH level is detrimental to P. olivaceus fertilized eggs.