Alterations of Mitochondrial Protein Assembly and Jasmonic Acid Biosynthesis Pathway in Honglian (HL)-type Cytoplasmic Male Sterility Rice
Alterations of Mitochondrial Protein Assembly and Jasmonic Acid Biosynthesis Pathway in Honglian (HL)-type Cytoplasmic Male Sterility Rice
复制标题
红莲型细胞质雄性不育水稻线粒体蛋白组装和茉莉酸生物合成途径的改变
DOI：
10.1074/jbc.m112.382549
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发表时间：
2012-11-16
影响因子：
4.8
通讯作者：
Zhu, Ying-Guo
中科院分区：
文献类型：
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作者：
Liu, Gai;Tian, Han;Zhu, Ying-Guo
有研究认为，线粒体嵌合基因orfH79是导致红莲细胞质雄性不育水稻小孢子败育的原因，但其作用机制尚不清楚。在这项研究中，我们使用基于质谱的定量蛋白质组学策略比较了不育系悦泰a与其可育近等基因系悦泰B的线粒体蛋白质组。我们发现，与悦泰B相比，悦泰a线粒体复合物中特定蛋白质的数量减少，这表明不育系线粒体复合物组装存在缺陷。Western blotting显示ORFH79蛋白和ATP1蛋白（复合体V的F-1部分成分）都与大小相似的大蛋白复合体相关。呼吸复合体活性测定和透射电镜显示，与悦泰b相比，悦泰A的线粒体在功能和形态上存在缺陷。此外，我们发现悦泰A的一个性别决定tasselseed2样蛋白增加，导致在小孢子发育过程中发现茉莉酸途径的异常变异。
It has been suggested that the mitochondrial chimeric gene orfH79 is the cause for abortion of microspores in Honglian cytoplasmic male sterile rice, yet little is known regarding its mechanism of action. In this study, we used a mass spectrometry-based quantitative proteomics strategy to compare the mitochondrial proteome between the sterile line Yuetai A and its fertile near-isogenic line Yuetai B. We discovered a reduced quantity of specific proteins in mitochondrial complexes in Yuetai A compared with Yuetai B, indicating a defect in mitochondrial complex assembly in the sterile line. Western blotting showed that ORFH79 protein and ATP1 protein, an F-1 sector component of complex V, are both associated with large protein complexes of similar size. Respiratory complex activity assays and transmission electron microscopy revealed functional and morphological defects in the mitochondria of Yuetai A when compared with Yuetai B. In addition, we identified one sex determination TASSELSEED2-like protein increased in Yuetai A, leading to the discovery of an aberrant variation of the jasmonic acid pathway during the development of microspores.