Immunoteratology of chlordane: cell-mediated and humoral immune responses in adult mice exposed in utero.
Immunoteratology of chlordane: cell-mediated and humoral immune responses in adult mice exposed in utero.
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氯丹的免疫畸胎学：子宫内暴露的成年小鼠的细胞介导和体液免疫反应。
DOI：
10.1016/0041-008x(82)90141-7
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发表时间：
1982
影响因子：
3.8
通讯作者：
M. Cranmer
中科院分区：
文献类型：
--
作者：
Joan M. Spyker;J. Barnett;D. Avery;M. Cranmer
通过评估细胞介导的和体液免疫应答，评价了产前暴露于氯代烃农药杀虫双的成年小鼠的免疫系统的功能状态。妊娠BALB c小鼠在整个妊娠期间每天喂食0、0.16或8.0 mg/kg的氯霉素，产生了存活的、明显正常的后代。在101日龄时，通过对恶唑酮的接触性超敏反应来测量细胞介导的免疫（CMI）反应。通过将恶唑酮应用于裸露的侧腹使后代致敏，并在5天后通过将恶唑酮经皮应用于耳进行激发。在激发后3天，通过每日测微计测量耳厚度来确定硬结程度。与对照组或较低剂量组的后代相比，较高剂量组的后代具有显著（p < 0.01）降低的CMI反应。在101日龄时使用溶血空斑试验测定对绵羊红细胞（sRBC）接种的主要体液反应。用洗涤的sRBC免疫受试者。通过加入补体（导致sRBC溶解）检测脾细胞释放的IgM抗体。从氯丹处理组或溶剂对照组的脾细胞产生的空斑形成细胞（PFC）的数量之间没有显着差异。本研究的结果揭示了两个显著的发现：（1）在明显正常的处理后代中，功能性CMI反应严重抑制，但（2）对T细胞依赖性体液免疫反应没有影响。氯霉素对CMI和PFC反应的影响可能是通过减少T效应细胞的活性或数量来解释的。
The functional status of the immune system of adult mice prenatally exposed to the chlorinated hydrocarbon pesticide chlordane was evaluated by assessing cell-mediated and humoral immune responses. Gravid BALB c mice fed 0, 0.16, or 8.0 mg/kg chlordane daily throughout gestation produced viable, overtly normal offspring. At 101 days of age cell-mediated immune (CMI) responses were measured by the contact hypersensitivity response to oxazolone. Offspring were sensitized by application of oxazolone to the denuded flank and 5 days later challenged by percutaneous application of oxazolone to the ear. Degree of induration was determined by daily micrometer measurement of ear thickness for 3 days after challenge. Offspring in the higher dose group had a significantly (p < 0.01) depressed CMI response when compared to offspring of the control or lower dose groups. The primary humoral response to sheep red blood cell (sRBC) innoculation was determined at 101 days of age using the hemolytic plaque assay. Subjects were immunized with washed sRBC. IgM antibody released from the spleen cells was detected by addition of complement which caused lysis of the sRBC's. No significant differences existed between the number of plaque-forming cells (PFC) produced by spleen cells from either chlordane-treated group or the vehicle-control group. Results of this study revealed two significant findings: (1) severe depression of the functional CMI response in apparently normal treated offspring, but (2) no effect on the T-cell-dependent humoral immune response. The effect of chlordane on CMI and PFC responses might be explained by a reduction in the activity or number of T effector cells.