Study on the effects of cefotaxime on intracellular Ca2+ in human peripheral lymphocytes by fluoremetry
Study on the effects of cefotaxime on intracellular Ca2+ in human peripheral lymphocytes by fluoremetry
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荧光法研究头孢噻肟对人外周淋巴细胞胞内Ca2+的影响
DOI：
10.1016/j.cclet.2006.12.044
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发表时间：
2007-04
期刊：
影响因子：
--
通讯作者：
Shuang, Shao Min
中科院分区：
文献类型：
--
作者：
Zhao, Chun Gui;Wang, Hai Yan;Zhou, Ye Hong;Wang, Dan Dan;Dong, Chuan;Shuang, Shao Min
利用荧光技术研究了Fura-2与Ca ~（2+）的相互作用特性。测定了不同温度下Fura-2-Ca ~（2+）络合物的表观解离常数（Kd）。以荧光染料Fura-2为探针，研究了头孢噻肟（CEFA）对细胞内Ca ~（2+）浓度（[Ca ~（2+）]i）的影响。静息状态下人外周血淋巴细胞[Ca ~（2+）] i为100± 7 nmol/L，经头孢噻肟处理后，在不同条件下观察到[Ca ~（2+）] i的变化。在1-30μmol/L头孢噻肟浓度范围内，胞内[Ca ~（2+）] i增加，这是由于胞内Ca ~（2+）库释放所致。高浓度（50-500μmol/L）的头孢噻肟可使细胞内[Ca ~（2+）]i降低。
Characteristic of Fura-2-Ca2+interaction was studied based on the fluorescence technique. The apparent dissociation constants (Kd) of the Fura-2-Ca2+complex were determined at different temperature. The effect of cefotaxime (CEFA) on intracellular Ca2+concentration ([Ca2+]i) was discussed by using a ratiometric fluorescence dye Fura-2 as a probe. The basal [Ca2+]iin resting human peripheral lymphocytes was 100±7nmol/L but after treatment with cefotaxime, the changes of [Ca2+]iwere observed in different conditions. In the concentration range of 1–30μmol/L of cefotaxime [Ca2+]iincreased, as a result of releasing intracellular Ca2+stores. Higher concentration of cefotaxime (50–500μmol/L) stimulated to decrease of [Ca2+]i.