COHABITATION OF SCAFFOLD BINDING REGIONS WITH UPSTREAM ENHANCER ELEMENTS OF 3 DEVELOPMENTALLY REGULATED GENES OF DROSOPHILA-MELANOGASTER
COHABITATION OF SCAFFOLD BINDING REGIONS WITH UPSTREAM ENHANCER ELEMENTS OF 3 DEVELOPMENTALLY REGULATED GENES OF DROSOPHILA-MELANOGASTER
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DOI：
10.1016/0092-8674(86)90877-9
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发表时间：
1986-08-15
期刊：
影响因子：
64.5
通讯作者：
LAEMMLI, UK
中科院分区：
文献类型：
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作者：
GASSER, SM;LAEMMLI, UK
我们发现DNA片段连接到核支架（SAR）的5“和3”的三个果蝇基因，定义环域范围从4.5到13 kb。对于含有两个启动子的基因Adh（乙醇脱氢酶），我们发现两个上游和两个下游SAR。对于Sgs-4，5 '' SAR覆盖转录物上游紧邻的866 bp，而对于fushi tarazu，5 '' SAR位于转录开始上游4.8 kb的小片段上。这四个上游支架连接片段与增强子样调控序列互补。五个上游SAR的序列分析揭示了与拓扑异构酶II的切割共有序列密切相关的序列簇，特定的10 bp富含A的序列的几个拷贝。图形 **。和另一个10 bp富含T的片段。
We find DNA fragments attached to the nuclear scaffold (SARs) both 5'' and 3'' of three Drosophila genes, defining looped domains ranging from 4.5 to 13 kb. For the two-promoter-containing gene Adh (alcohol dehydrogenase), we find two upstream and two downstream SARs. For Sgs-4, the 5'' SAR covers 866 bp immediatedly upstream of the transcript, and in the case of fushi tarazu, the 5'' SAR is found on a small fragment 4.8 kb upstream of the start of transcription. These four upstream scaffold-attached fragments comap with enhancer-like regulatory sequences. Sequence analysis of five upstream SARs reveals clusters of sequences closely related to the cleavage consensus of topoisomerase II, several copies of a specific 10 bp A-rich sequence .**GRAPHIC**. and another 10 bp T-rich stretch.