Newcastle disease virus (NDV) recombinant expressing the hemagglutinin of H7N9 avian influenza virus protects chickens against NDV and highly pathogenic avian influenza A (H7N9) virus challenges
Newcastle disease virus (NDV) recombinant expressing the hemagglutinin of H7N9 avian influenza virus protects chickens against NDV and highly pathogenic avian influenza A (H7N9) virus challenges
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DOI：
10.1016/j.vaccine.2017.10.010
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发表时间：
2017-12-04
期刊：
影响因子：
5.5
通讯作者：
Liu, Xiufan
中科院分区：
文献类型：
--
作者：
Hu, Zenglei;Liu, Xiaowen;Liu, Xiufan
中国新出现的高致病性甲型H7N9禽流感病毒对公共卫生和家禽养殖业构成了双重挑战。因此，迫切需要H7N9疫苗。在本研究中，我们构建了表达H7N9病毒血凝素(HA)的新城疫病毒(NDV)载体疫苗(RLXHAF)，并将其与新城疫病毒融合蛋白的跨膜/胞质尾区融合。RLXHAF稳定表达HA蛋白，表现出与亲本病毒相似的生长动力学和致病性。RLXHAF能诱导出NDV特异性血凝抑制(HI)、病毒中和抗体(VN)和总IgY抗体，并能完全保护雏鸡免受新城疫病毒攻击。出乎意料的是，rLXHAF产生了无法检测到的HI和VN效价，但通过ELISA检测，抗H7N9的总IgY抗体效价很高。该疫苗对HPAI H7N9攻击的保护率为80%。新城疫病毒和H7N9攻毒株的病毒脱落减少。结果表明，rLXHAF具有免疫原性，对HPAI H7N9病毒具有较强的免疫原性。(C)2017爱思唯尔有限公司。保留所有权利。
The emerged highly pathogenic avian influenza A (H7N9) (HPAI) viruses in China pose a dual challenge to public health and poultry industry. Thus H7N9 vaccines are in an urgent need. In this study, we constructed a Newcastle disease virus (NDV)-vectored vaccine (rLXHAF) expressing the hemagglutinin (HA) of H7N9 virus fused with the transmembrane/cytoplasmic tail domain of the NDV fusion protein. rLXHAF stably expressed the HA protein, exhibited similar growth kinetics and pathogenicity as the parental virus. rLXHAF induced positive NDV-specific hemagglutination inhibition (HI), virus neutralization (VN) and total IgY antibodies and completely protected chickens from NDV challenge. Unexpectedly, rLXHAF elicited undetectable HI and VN titers but high overall IgY antibody titers against H7N9 measured by ELISA. The vaccine provided 80% protection against HPAI H7N9 challenge. Virus shedding of NDV and H7N9 challenge strains was reduced. Our results suggest that rLXHAF is immunogenic and efficacious against HPAI H7N9 virus in chickens. (C) 2017 Elsevier Ltd. All rights reserved.