Oligomycin induced the proteasomal degradation of cyclin D1 protein
Oligomycin induced the proteasomal degradation of cyclin D1 protein
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DOI：
10.1038/ja.2009.47
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发表时间：
2009-08-01
影响因子：
3.3
通讯作者：
Imoto, Masaya
中科院分区：
文献类型：
--
作者：
Kanai, Mai;Iba, Satoru;Imoto, Masaya
我们使用带有Cre/ loxp调节的cyclin D1过表达系统的重组腺病毒，从内部天然产物文库中寻找影响cyclin D1/视网膜母细胞瘤蛋白通路的化合物，我们发现寡霉素在cyclin D1过表达的SW480细胞中比在对照SW480细胞中更有效地抑制细胞生长。我们还发现，寡霉素降低了cyclin D1蛋白的表达水平，这种降低至少部分是由Thr-286磷酸化依赖性蛋白酶体降解介导的。抗生素杂志（2009）62,425-429；doi: 10.1038 / ja.2009.47;2009年7月3日在线发表
We searched for compounds that affect the cyclin D1/retinoblastoma protein pathway from the in-house natural product library using a recombinant adenovirus with the Cre/loxP-regulated cyclin D1 overexpression system, and we found that oligomycin inhibited cell growth more effectively in cyclin D1-overexpressing SW480 cells than in control SW480 cells. We also found that oligomycin reduced the expression levels of cyclin D1 protein and that this reduction is, at least in part, mediated by Thr-286 phosphorylation-dependent proteasomal degradation. The Journal of Antibiotics (2009) 62, 425-429; doi: 10.1038/ja.2009.47; published onl ine 3 July 2009