Adsorption Behavior of Microbes on a QCM Chip Modified with an Artificial Siderophore-Fe3+ Complex
Adsorption Behavior of Microbes on a QCM Chip Modified with an Artificial Siderophore-Fe3+ Complex
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DOI：
10.1021/la203250n
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发表时间：
2012-01-17
期刊：
影响因子：
3.9
通讯作者：
Masuda, Hideki
中科院分区：
文献类型：
--
作者：
Inomata, Tomohiko;Eguchi, Hiroshi;Masuda, Hideki
合成了三种末端带有NH_2基团的异羟肟酸型人工铁载体三[2-{3-（N-酰基-N-羟氨基）丙酰胺基}-丙基]氨基甲烷（1-3，酰基-R基团分别为Me、Et和Ph）及其Fe ~（3+）配合物4-6。通过EDTA滴定，估计4的稳定常数（log beta）约为31。4-6对微杆菌（Microbacterium flavescens）的生物活性清楚地表明，它们依赖于其末端庞大的酰基-R基团而渗透细胞膜，微杆菌是一种异羟肟酸盐型铁载体、营养缺陷型革兰氏阳性微生物。将这些人工铁载体复合物4-6用末端NH 2基团修饰在Au电极表面上（4-6/Au）。4-6的表面改性通过若干电化学测量证实。石英晶体微天平（QCM）芯片也用4-6.利用这些改进的QCM芯片对M. flavescens、恶臭假单胞菌和大肠埃希菌。QCM芯片具有选择性吸附微生物的能力，这是由于Fe 3 +-人工铁载体复合物与其细胞膜内的受体或结合蛋白之间的相互作用的结构差异。
Three hydroxamate-type artificial siderophores with terminal NH2 groups, tris[2-{3-(N-acyl-N-hydroxamino)propylamido}-propyl]aminomethane (1-3, acyl-R group = Me, Et, and Ph, respectively), and their Fe3+ complexes, 4-6, were prepared. The stability constant (log beta) of 4 was estimated to be about 31 by its EDTA titration. The biological activities of 4-6 for Microbacterium flavescens, which is a hydroxamate-type siderophore, auxotrophic gram-positive microbe, clearly indicated that they permeated the cell membrane depending on their terminal bulky acyl-R groups. These artificial siderophore complexes, 4-6, were modified on Au electrode surfaces with the terminal NH2 group (4-6/Au). The surface modification of 4-6 was confirmed by several electrochemical measurements. The quartz crystal microbalance (QCM) chips were also modified with 4-6. Microbe adsorption measurements using these modified QCM chips for M. flavescens, Pseudomonas putida, and Eschrichia coli were performed. The QCM chips have the ability to adsorb microbes selectively as a result of the differences in the interactions between the structures of Fe3+-artificial siderophore complexes and their receptors or binding proteins within the cell membrane.