Prevalence of salmonellae, Listeria monocytogenes, and fecal coliforms in bulk tank milk on US dairies
Prevalence of salmonellae, Listeria monocytogenes, and fecal coliforms in bulk tank milk on US dairies
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DOI：
10.3168/jds.s0022-0302(04)73410-4
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发表时间：
2004-09-01
影响因子：
3.5
通讯作者：
Perdue, ML
中科院分区：
文献类型：
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作者：
Van Kessel, JS;Karns, JS;Perdue, ML
本研究的目的是确定美国散装罐奶中沙门氏菌、单核细胞增生李斯特菌和粪大肠杆菌的患病率。作为NAHMS乳制品2002年调查的一部分，从21个州的农场收集了861个散装罐牛奶样本。将牛奶直接铺在选择性琼脂上进行直接细菌计数，并在选择性肉汤中富集以增加检测灵敏度。还测定了体细胞计数（SCC）和标准平板计数（SPC）。在95%（860份中的818份）的样本中检测到大肠菌群，平均SCC为295，000个细胞/mL。22个样本（2.6%）为沙门氏菌培养阳性，鉴定出9种血清型：蒙得维的亚（n = 7）、纽波特（n = 4）、明斯特（n = 2）、Meleagridis（n = 2）、Cerro（n = 2）、44：Z36（Z38）（n = 2）、都柏林（n = 1）、Anatum（n = 1）和9、12：无运动（n = 1）。从56份（6.5%）样品中分离出单核细胞增生李斯特菌，这些分离株的血清分型产生5种血清型（1/2a，1/2b，3b，4 b和4c）。关于L.单核细胞增生分离株中，93%为血清型1/2a、1/ 2b和4 b，这是最常见的人类临床分离株。L.的区域差异单核细胞增多症和沙门氏菌的患病率，但需要更多的研究来确定这些差异的有效性。SCC和SPC与沙门氏菌和L.单核细胞增多症虽然L.单核细胞增生症和沙门氏菌的含量较低，这些病原体对生奶和生奶制品的消费者构成潜在风险。
The objective of this study was to determine the prevalence of Salmonella, Listeria monocytogenes, and fecal coliforms in bulk tank milk in the United States. As part of the NAHMS Dairy 2002 survey, 861 bulk tank milk samples were collected from farms in 21 states. Milk was directly plated on selective agars for direct bacterial enumeration and was enriched in selective broths to increase detection sensitivity. Somatic cell counts (SCC) and standard plate counts (SPC) were also determined. Coliforms were detected in 95% ( 818 of 860) of the samples, and the average SCC was 295,000 cells/mL. Twenty-two samples (2.6%) were culture-positive for Salmonella, and 9 serotypes were identified: Montevideo (n = 7), Newport ( n = 4), Muenster ( n = 2), Meleagridis ( n = 2), Cerro ( n = 2), 44: Z36 (Z38) ( n = 2), Dublin ( n = 1), Anatum ( n = 1), and 9, 12: nonmotile ( n = 1). Listeria monocytogenes was isolated from 56 (6.5%) samples, and serotyping of these isolates yielded 5 serotypes (1/2a, 1/2b, 3b, 4b, and 4c). Of the L. monocytogenes isolates, 93% were serotypes 1/2a, 1/ 2b, and 4b, the most common human clinical isolates. Regional differences in L. monocytogenes and Salmonella prevalence were observed, but more studies are needed to determine the validity of these differences. There were no apparent relationships between SCC or SPC and incidence of Salmonella or L. monocytogenes. Although the prevalence of L. monocytogenes and Salmonella was low, these pathogens represent a potential risk to consumers of raw milk and raw milk products.