Cultivation of Trypanosoma cruzi epimastigotes in low glucose axenic media shifts its competence to differentiate at metacyclic trypomastigotes
Cultivation of Trypanosoma cruzi epimastigotes in low glucose axenic media shifts its competence to differentiate at metacyclic trypomastigotes
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DOI：
10.1016/j.exppara.2008.03.003
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发表时间：
2008-07-01
影响因子：
2.1
通讯作者：
Contreras, Victor T.
中科院分区：
文献类型：
--
作者：
De Lima, Ana R.;Navarroa, Maria C.;Contreras, Victor T.
这项研究揭示了为什么克氏锥虫上鞭毛体如果通过连续传代长期维持在培养基中，会失去分化为后循环形式的能力。比较了从不同来源（人类、负鼠）分离并维持在两种方案（交替锥蝽/小鼠传代和连续培养基）下的两种克氏锥虫菌株的生物学和代谢行为。为了确定环境对寄生虫的影响，将上鞭毛体在极端条件（高和低葡萄糖浓度）下生长，并比较一个隔室（沿着生长曲线）或两个隔室（诱导后生发育）的葡萄糖消耗、氨产生和 pH 值变化。还评估了葡萄糖对抗原水平上的后生发育阶段的影响。结果表明，克氏锥虫能够适应各种环境条件，并且上鞭毛体进行后生发育的能力受到维持计划的影响。抗原谱分析支持这样的观点，即适应培养基的上鞭毛体并未完成其分子分化至锥鞭毛体后循环阶段。这些过渡形式在一定程度上保留了上鞭毛体阶段的基因表达。 (c) 2008 Elsevier Inc. 保留所有权利。
This study offers an insight into why Trypanosoma cruzi epimastigotes lose their capacity to differentiate into metacyclic forms, if maintained in culture media long-term through serial passages. The biological and metabolic behaviour of two T. cruzi strains isolated from various origins (human, opossum), and maintained under two schedules (alternate triatomine/mouse passages and serial culture media) were compared. To determine the effect of the environment on the parasites, the epimastigotes were grown under extreme conditions (high and low glucose concentrations), and the glucose consumption, ammonia production and changes in pH, either in one compartment (along the growth curve) or two compartments (induced metacyclogenesis) were compared. The glucose effect on the stages involved in metacyclogenesis at antigenic level was also evaluated. The results indicate that T cruzi adapts to various environmental conditions and also that the ability of epimastigotes to undergo metacyclogenesis are influenced by the maintenance schedule. Antigenic profile analysis supports the idea that epimastigotes adapted to culture media do not complete their molecular differentiation into the trypomastigote metacyclic stage. These transition forms conserve some degree of gene expression of the epimastigote stage. (c) 2008 Elsevier Inc. All rights reserved.