BINDING OF HMG-17 TO MONONUCLEOSOMES OF THE AVIAN BETA-GLOBIN GENE-CLUSTER IN ERYTHROID AND NON-ERYTHROID CELLS
BINDING OF HMG-17 TO MONONUCLEOSOMES OF THE AVIAN BETA-GLOBIN GENE-CLUSTER IN ERYTHROID AND NON-ERYTHROID CELLS
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DOI：
10.1093/nar/18.8.2011
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发表时间：
1990-04-25
影响因子：
14.9
通讯作者：
GINDER, GD
中科院分区：
文献类型：
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作者：
BROTHERTON, TW;RENEKER, J;GINDER, GD
hmg17与含有禽流感DNA的剥离核小体的结合。我们检查了-珠蛋白基因簇，以确定在体外结合在这个发育调节的基因区域是否与完整细胞核的转录活性或DNAse i敏感性有关。分别从鸡胚的原始期和终末期胚胎红细胞、成体网织红细胞和成体网织红细胞中制备单核小体。诱导-珠蛋白转录，成年胸腺细胞。hmg17优先与含有。β的单核小体结合。-珠蛋白基因簇局限于含有胚胎红细胞的单核小体。-珠蛋白基因，成人。-珠蛋白基因，以及位于这两个基因之间的DNA序列，但不包括那些含有胚胎- epsilon的基因。球蛋白基因。将这些结果与已知的转录模式和dna酶i敏感性进行比较。-珠蛋白基因簇表明，HMG - 17的结合虽然具有组织特异性，但与dna酶i敏感性或活性基因转录没有直接关系，而是依赖于该活性基因结构域核心单核小体中存在的其他因素。
The binding of HMG 17 to stripped core mononucleosomes containing DNA from the avian .beta.-globin gene cluster was examined to determine whether binding in vitro in this developmentally-regulated gene domain was associated with transcriptional activity or DNAse I-sensitivity in intact nuclei. Mononucleosomes were prepared from primitive and definitive stage embryonic red bloods cells of chick embryos, adult reticulocytes, adult reticulocytes in which embryonic .rho.-globin transcription was induced, and adult thymus cells. Preferential binding by HMG 17 to mononucleosomes containing the .beta.-globin gene cluster was confined to erythroid-derived mononucleosomes that contain the embryonic .rho.-globin gene, the adult .beta.-globin gene, and DNA sequences located between these two genes, but not to those that contain the embryonic .epsilon.-globin gene. Comparison of these results to the known patterns of transcription and DNase I-sensitivity within the .beta.-globin gene cluster shows that HMG 17 binding, although tissue-specific, does not correlate directly with either DNase I-sensitivity or active gene transcription, but is dependent on other factors present in core mononucleosomes from this active gene domain.