Both T and B cells shed infectious mouse mammary tumor virus
Both T and B cells shed infectious mouse mammary tumor virus
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DOI：
10.1128/jvi.71.8.6044-6048.1997
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发表时间：
1997-08-01
影响因子：
5.4
通讯作者：
Ross, SR
中科院分区：
文献类型：
--
作者：
Dzuris, JL;Golovkina, TV;Ross, SR
小鼠乳腺肿瘤病毒（MMTV）经乳传播后，在体内感染B、T组织培养细胞和原代B、T细胞。受感染的组织培养细胞处理病毒蛋白，这些细胞和原代B细胞和T细胞在体外培养时脱落病毒。此外，感染的B和T组织培养细胞在体外将病毒传播给未感染的乳腺细胞，这些不同类型细胞在体内的感染水平取决于小鼠的品系，C3H/HeN小鼠在mmtv感染的C3H/HeN母亲的哺乳后表现出更大的B细胞感染，BALB/c小鼠表现出更大的T细胞感染，尽管它们的B细胞感染较少。BALB/c小鼠比C3H/HeN小鼠发生肿瘤的速度更快，这些结果表明感染的T细胞和B细胞都是体内MMTV的潜在携带者。
Mouse mammary tumor virus (MMTV) infected both B and T tissue culture cells and primary B and T cells in vivo after milk-borne transmission of the virus. The infected tissue culture cells processed, viral proteins, and both these and primary B and T cells shed virus when cultured in vitro. Moreover, the infected B and T tissue culture cells transmitted virus to uninfected mammary gland cells in vitro, The level of infection of these different cell types in vivo was dependent on the strain of mouse, with C3H/HeN mice showing greater B-cell infection and BALB/c mice greater T-cell infection after nursing on MMTV-infected C3H/HeN mothers, Although their B cells were less infected. BALB/c mice developed tumors more rapidly than C3H/HeN mice, These results indicate that both infected T and B cells are potential carriers of MMTV in vivo.