An integration-free iPSC line (SDQLCHi012-A) derived from a patient with inflammatory bowel disease-28 carrying compound heterozygote mutations in IL10RA gene
An integration-free iPSC line (SDQLCHi012-A) derived from a patient with inflammatory bowel disease-28 carrying compound heterozygote mutations in IL10RA gene
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源自患有炎症性肠病 28 的患者的无整合 iPSC 系 (SDQLCHi012-A)，该患者携带 IL10RA 基因复合杂合子突变
DOI：
10.1016/j.scr.2019.101577
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发表时间：
2019
影响因子：
1.2
通讯作者：
Yi Liu
中科院分区：
文献类型：
--
作者：
Ma Yanyan;Zhang Haiyan;Zhang Shisong;Dong Rui;Yang Xiaomeng;Li Yue;Guan Jingyun;Gai Zhongtao;Yi Liu
诱导性多能干细胞（iPSC）系（SDQLCHi 012-A）产生自一名11个月大的男性的外周血单核细胞，该男性被诊断为由IL 10 RA突变（c.188 + 1G > A和c.301C > T）的复合杂合子引起的炎性肠病-28。编码0 CT 4、S 0X 2、KLF 4、BCL-XL和MYC的非整合附加型载体用于重编程。所建立的iPSC系含有在患者中鉴定的相同突变，显示正常核型、体外分化潜力和表达的多能性标记物。
Induced pluripotent stem cell (iPSC) line (SDQLCHi012-A) was generated from peripheral blood mononuclear cells of an 11-month-old male who was diagnosed as inflammatory bowel disease-28 caused by compound heterozygote forIL10RAmutations (c.188 + 1G > A and c.301C > T). Non-integrating episomal vectors coding OCT4, SOX2, KLF4, BCL-XL and MYC were used for reprogramming. The established iPSC line contained the same mutations identified in the patient, showed a normal karyotype, differentiation potential in vitro and expressed pluripotency markers.