The R1 gene for potato resistance to late blight (Phytophthora infestans) belongs to the leucine zipper/NBS/LRR class of plant resistance genes
The R1 gene for potato resistance to late blight (Phytophthora infestans) belongs to the leucine zipper/NBS/LRR class of plant resistance genes
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DOI：
10.1046/j.1365-313x.2001.01292.x
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发表时间：
2002-05-01
期刊：
影响因子：
7.2
通讯作者：
Gebhardt, C
中科院分区：
文献类型：
--
作者：
Ballvora, A;Ercolano, MR;Gebhardt, C
马铃薯晚疫病菌(Phytophthora Infestans)是马铃薯栽培中最具破坏性的病害。新的、毒力更强的致病疫霉菌株已经进化出来，克服了从野生马铃薯品种到商业品种的传统育种引入的遗传抗性。野生和栽培马铃薯种质中均存在R基因(单基因抗性)和数量抗晚疫病基因。晚疫病的单基因和数量抗性的分子基础尚不清楚。我们通过定位克隆和候选基因相结合的方法克隆了第一个抗晚疫病基因R1。R1基因是一个基因家族的成员。它编码1,293个氨基酸的蛋白质，分子质量为149.4 kDa。R1基因属于植物抗病基因的一类，具有亮氨酸拉链基序、核苷酸结合区和富含亮氨酸的重复结构域。与番茄抗性关系最密切的植物抗性基因(同源性为36%)是番茄抗病基因prf。R1位于马铃薯V染色体上的抗病热点区域，与感病等位基因相比，其抗性等位基因代表了一个功能R基因的大插入。
Late blight caused by the oomycete Phytophthora infestans is the most destructive disease in potato cultivation worldwide. New, more virulent P. infestans strains have evolved which overcome the genetic resistance that has been introgressed by conventional breeding from wild potato species into commercial varieties. R genes (for single-gene resistance) and genes for quantitative resistance to late blight are present in the germplasm of wild and cultivated potato. The molecular basis of single-gene and quantitative resistance to late blight is unknown. We have cloned R1, the first gene for resistance to late blight, by combining positional cloning with a candidate gene approach. The R1 gene is member of a gene family. It encodes a protein of 1293 amino acids with a molecular mass of 149.4 kDa. The R1 gene belongs to the class of plant genes for pathogen resistance that have a leucine zipper motif, a putative nucleotide binding domain and a leucine-rich repeat domain. The most closely related plant resistance gene (36% identity) is the Prf gene for resistance to Pseudomonas syringae of tomato. R1 is located within a hot spot for pathogen resistance on potato chromosome V. In comparison to the susceptibility allele, the resistance allele at the R1 locus represents a large insertion of a functional R gene.