Analysis of gene expression and regulation implicates C2H9orf152 has an important role in calcium metabolism and chicken reproduction
Analysis of gene expression and regulation implicates C2H9orf152 has an important role in calcium metabolism and chicken reproduction
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基因表达和调控分析表明 C2H9orf152 在钙代谢和鸡繁殖中具有重要作用
DOI：
10.1016/j.anireprosci.2016.11.002
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发表时间：
2017-01-01
影响因子：
2.2
通讯作者：
Ning, Zhonghua
中科院分区：
文献类型：
--
作者：
Liu, Long;Fan, Yanfeng;Ning, Zhonghua
雌鸟的生殖系统负责产蛋。在输卵管中高表达的基因具有潜在的重要性。 RNA-seq分析表明，C2H9orf152（人C9orf152的直系同源基因）在鸡子宫中高表达。为了推断其功能，我们获得并表征了其完整的 cDNA 序列，确定了其时空表达，并通过药物方法探测了其转录因子。数据显示，完整的cDNA序列长1468 bp，开放阅读框789 bp。与其他测试组织相比，该基因在输卵管和肝脏组织中高表达，尤其是子宫。在发育和生殖时期其在子宫中的表达逐渐增加，证实了其参与生殖系统的生长和成熟。相反，其表达在活跃子宫和静止子宫之间没有显着差异，这表明 C2H9orf152 在生殖中的作用可能是由于其长期效应。此外，根据其5'-侧翼序列，Foxd3和Hnf4a被预测为C2H9orf152的转录因子。使用小檗碱或视黄酸（分别可以调节 Hnf4a 和 Foxd3 的活性），我们证明了鸡原代肝细胞中的化学物质对 C2H9orf152 的抑制作用。由于视黄酸调节钙代谢，而Hnf4a是肝脏的关键核因子，这些研究结果表明C2H9orf152参与肝功能和生殖系统的钙代谢。总之，C2H9orf152可能通过调节钙代谢对鸡生殖系统产生长期影响，表明该基因对提高产蛋量和蛋壳质量具有重要意义。 (C) 2016 Elsevier B.V. 保留所有权利。
The reproductive system of a female bird is responsible for egg production. The genes highly expressed in oviduct are potentially important. From RNA-seq analysis, C2H9orf152 (an orthologous gene of human C9orf152) was identified as highly expressed in chicken uterus. To infer its function, we obtained and characterized its complete cDNA sequence, determined its spatiotemporal expression, and probed its transcription factor(s) through pharmaceutical approach. Data showed that the complete cDNA sequence was 1468 bp long with a 789 bp of open reading frame. Compared to other tested tissues, this gene was highly expressed in the oviduct and liver tissues, especially uterus. Its expression in uterus was gradually increased during developmental and reproductive periods, which verified its involvement in the growth and maturity of reproductive system. In contrast, its expression was not significant different between active and quiescent uterus, suggesting the role of C2H9orf152 in reproduction is likely due to its long-term effect. Moreover, based on its 5'- flanking sequence, Foxd3 and Hnf4a were predicted as transcription factors of C2H9orf152. Using berberine or retinoic acid (which can regulate the activities of Hnf4a and Foxd3, respectively), we demonstrated suppression of C2H9orf152 by the chemicals in chicken primary hepatocytes. As retinoic acid regulates calcium metabolism, and Hnf4a is a key nuclear factor to liver, these findings suggest that C2H9orf152 is involved in liver function and calcium metabolism of reproductive system. In conclusion, C2H9orf152 may have a long-term effect on chicken reproductive system by regulating calcium metabolism, suggesting this gene has an important implication in the improvement of egg production and eggshell quality. (C) 2016 Elsevier B.V. All rights reserved.