A radiation hybrid map of the human genome
A radiation hybrid map of the human genome
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DOI：
10.1093/hmg/5.3.339
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发表时间：
1996-03-01
影响因子：
3.5
通讯作者：
Goodfellow, PN
中科院分区：
文献类型：
--
作者：
Gyapay, G;Schmitt, K;Goodfellow, PN
我们通过将辐射后的二倍体人成纤维细胞与受体仓鼠细胞融合，开发了一组全基因组辐射杂交体。用已知遗传位置的微卫星标记对这组-168个细胞系进行了分型，其中711个AFM遗传标记404被用来构建一个覆盖所有常染色体和X染色体的稳健的框架图。为了证明该面板的实用性，将374个先前分配给第1、2、14和16号染色体的表达序列标签(EST)定位在该图谱上。所有这些EST都可以通过成对连接到LOD分数大于8的框架标记之一进行定位。这里描述的全基因组辐射杂交面板已被用作GeneBridge 4面板的起始材料，该面板正被广泛用于基因组图谱计划。
We have developed a panel of whole-genome radiation hybrids by fusing irradiated diploid human fibroblasts with recipient hamster cells. This panel of -168 cell lines has been typed with microsatellite markers of known genetic location, Of 711 AFM genetic markers 404 were selected to construct a robust framework map that spans all the autosomes and the X chromosome. To demonstrate the utility of the panel, 374 expressed sequence tags (ESTs) previously assigned to chromosomes 1, 2, 14 and 16 were localized on this map. All of these ESTs could be positioned by pairwise linkage to one of the framework markers with a LOD score of greater than 8. The whole genome radiation hybrid panel described here has been used as the starting material for the Genebridge4 panel that is being made widely available for genome mapping projects.