Interactions between lens epithelial and fiber cells reveal an intrinsic self-assembly mechanism.
Interactions between lens epithelial and fiber cells reveal an intrinsic self-assembly mechanism.
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DOI：
10.1016/j.ydbio.2013.10.030
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发表时间：
2014-01-15
影响因子：
2.7
通讯作者：
McAvoy, J. W.
中科院分区：
文献类型：
--
作者：
Dawes, L. J.;Sugiyama, Y.;Lovicu, F. J.;Harris, C. G.;Shelley, E. J.;McAvoy, J. W.
关键词：
组织和器官如何发育并维持其特有的三维细胞结构，在其发育过程中往往是一个知之甚少的部分;然而，正如眼睛透镜的情况一样，这些特征的精确调节对功能至关重要。在透镜形态发生期间，细胞被组织成极化的球形结构，单层上皮细胞覆盖在细长纤维细胞的顶端。上皮细胞增殖，转移到透镜赤道线以下的后代分化成新的纤维，这些纤维逐渐加入纤维团。现在已知FGF诱导上皮细胞向纤维分化;然而，这两种形式的细胞如何组装成其特征性极化排列尚不完全清楚。在这里，我们表明，在FGF处理的上皮外植体，伸长的纤维成为极化/取向的上皮细胞的岛屿和模仿他们的极化排列在体内。上皮外植体分泌Wnt 5到培养基中，我们表明，Wnt 5可以促进定向行为的透镜细胞。我们还表明，这些外植体复制方面的Notch/锯齿状的信号传导活动，已被证明在体内调节上皮细胞的增殖。因此，我们的体外研究确定了一种新的机制，固有的两种形式的透镜细胞，有利于自组装成极化的安排特点的透镜在体内。以这种方式，透镜，其相对简单的细胞组成，作为一个有用的模型，以突出组织发育和再生过程中的这种内在的自组装机制的重要性。
How tissues and organs develop and maintain their characteristic three-dimensional cellular architecture is often a poorly understood part of their developmental program; yet, as is clearly the case for the eye lens, precise regulation of these features can be critical for function. During lens morphogenesis cells become organized into a polarized, spheroidal structure with a monolayer of epithelial cells overlying the apical tips of elongated fiber cells. Epithelial cells proliferate and progeny that shift below the lens equator differentiate into new fibers that are progressively added to the fiber mass. It is now known that FGF induces epithelial to fiber differentiation; however, it is not fully understood how these two forms of cells assemble into their characteristic polarized arrangement. Here we show that in FGF-treated epithelial explants, elongating fibers become polarized/oriented towards islands of epithelial cells and mimic their polarized arrangement in vivo. Epithelial explants secrete Wnt5 into the culture medium and we show that Wnt5 can promote directed behaviour of lens cells. We also show that these explants replicate aspects of the Notch/Jagged signaling activity that has been shown to regulate proliferation of epithelial cells in vivo. Thus, our in vitro study identifies a novel mechanism, intrinsic to the two forms of lens cells, that facilitates self-assembly into the polarized arrangement characteristic of the lens in vivo. In this way the lens, with its relatively simple cellular composition, serves as a useful model to highlight the importance of such intrinsic self-assembly mechanisms in tissue developmental and regenerative processes.
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影响因子：
2.7
作者：
Chen, Yongjuan;Stump, Richard J. W.;Lovicu, Frank J.;Shimono, Akihiko;McAvoy, John W.
通讯作者：
McAvoy, John W.
影响因子：
5.3
作者：
Jia, Junling;Lin, Min;Zhang, Pumin
通讯作者：
Zhang, Pumin
影响因子：
4.6
作者：
Lyu, J;Joo, CK
通讯作者：
Joo, CK
影响因子：
14.8
作者：
Chen, Baozhi;Dodge, Michael E.;Tang, Wei;Lu, Jianming;Ma, Zhiqiang;Fan, Chih-Wei;Wei, Shuguang;Hao, Wayne;Kilgore, Jessica;Williams, Noelle S.;Roth, Michael G.;Amatruda, James F.;Chen, Chuo;Lum, Lawrence
通讯作者：
Lum, Lawrence
影响因子：
4.4
作者：
Dawes, Lucy J.;Sugiyama, Yuki;McAvoy, John W.
通讯作者：
McAvoy, John W.