Probiotic treatment improves the impaired spatial cognitive performance and restores synaptic plasticity in an animal model of Alzheimer's disease
Probiotic treatment improves the impaired spatial cognitive performance and restores synaptic plasticity in an animal model of Alzheimer's disease
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DOI：
10.1016/j.bbr.2019.112183
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发表时间：
2019-12-30
影响因子：
2.7
通讯作者：
Salami, Mahmoud
中科院分区：
文献类型：
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作者：
Asl, Zahra Rezaei;Sepehri, Gholamreza;Salami, Mahmoud
研究表明，肠道微生物区系受损与一些大脑疾病有关，包括阿尔茨海默病(AD)等神经退行性疾病。因此，支持肠道微生物区系被认为是治疗AD的一种可能的策略。我们评估了脑室注射β-淀粉样蛋白造成的AD动物模型的行为和电生理方面的脑功能。两组对照组大鼠分别接受水作为载体(CON)或益生菌(Pro+CON)。另外，两组阿尔茨海默病动物分别接受赋形剂(ALZ)或益生菌(Pro+ALZ)治疗。假手术组只接受手术和接送车辆。用Morris水迷宫测试大鼠的空间学习记忆能力。通过记录海马场兴奋性突触后电位(FEPSP)来评估基础突触传递和长时程增强(LTP)。通过测定血浆总抗氧化能力(TAC)和丙二醛(MDA)水平来评价抗氧化/氧化因子的变化。进行脑部染色以确认β淀粉样蛋白的堆积。粪便细菌定量是为了找出益生菌补充剂对肠道微生物区系的影响。我们发现，虽然阿尔茨海默病动物表现出较弱的空间表现，但益生菌治疗改善了Pro+阿尔茨海默病大鼠的迷宫导航。虽然阿尔茨海默病大鼠的基本突触传递没有变化，但LTP在这组大鼠中受到抑制。益生菌治疗显著恢复了Pro+ALZ组大鼠的LTP，并进一步增强了Pro+CON大鼠的LTP。干预对Pro+ALZ大鼠体内抗氧化/氧化生物标志物的平衡也有良好的效果。本研究首次证明了益生菌对AD动物模型突触可塑性的积极作用。
Studies demonstrate that damage to gut microbiota is associated with some brain disorders including neurodegenerative diseases such as Alzheimer's disease (AD). Accordingly, supporting gut microbiota has been considered as a possible strategy for AD treatment. We evaluated behavioral and electrophysiological aspects of the brain function in an animal model of AD made by intracerebroventricular injection of beta-amyloid. Two groups of control rats recieved either water as vehicle (Con) or probitics (Pro + Con). Also two groups of Alzheimeric animals were treated by either vehicle (Alz) or probiotics (Pro + Alz). Sham group was only subjected to surgical procedure and received the vehicle. Spatial learning and memory was assessed in Morris water maze. Also, basic synaptic transmission and long-term potentiation (LTP) were assessed by recording field excitatory postsynaptic potentials (fEPSPs) in hippocampus. Change in anti-oxidant/oxidant factors was assessed via measuring plasma level of total anti-oxidant capacity (TAC) and malondealdehyde (MDA). Brain staining was done to confirm beta-amyloid accumulation. Fecal bacteria quantification was accomplished to find how probiotic supplement affected gut microbiota. We found that while the Alz animals displayed a weak spatial performance, probiotic treatment improved the maze navigation in the Pro + Alz rats. Whereas basic synaptic transmission remained unchanged in the Alz rats, LTP was suppressed in this group. Probiotic treatment significantly restored LTP in the Pro + Alz group and further enhanced it in the Pro + Con rats. The intervention also showed a favorable effect on balance of the anti-oxidant/oxidant biomarkers in the Pro + Alz rats. This study provides the first proof on positive effect of probiotics on synaptic plasticity in an animal model of AD.