Rapid and convenient synthesis of stable silver nanoparticles with kiwi juice and its novel application for detecting protease K
Rapid and convenient synthesis of stable silver nanoparticles with kiwi juice and its novel application for detecting protease K
复制标题
用猕猴桃汁快速便捷合成稳定的银纳米粒子及其在检测蛋白酶K中的新应用
DOI：
10.1039/c4nj01578a
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发表时间：
2015-01-01
影响因子：
3.3
通讯作者：
Wang, Jian
中科院分区：
文献类型：
--
作者：
Chen, Bin Bin;Liu, Hai;Wang, Jian
在这项工作中，猕猴桃汁作为还原剂和封端剂来获得稳定的银纳米颗粒（AgNP），这是非常有效且廉价的。重要的是，它可以在室温下 2 分钟内完成，使合成变得更快更容易。与最常见的AgNPs（例如柠檬酸钠包覆的AgNPs）相比，所提出的AgNPs表现出优异的性能，例如高稳定性。此外，建立了一种新的蛋白酶K分析方法，当将蛋白酶K引入AgNPs水溶液时，其颜色从浅黄色变为棕色。结果发现，AgNPs在470 nm处吸光度的增加与蛋白酶K浓度在0.50～15 mg·mL(-1)范围内成正比，检测限低至0.47 mg·mL(-1)。这种选择性比色测定法由于其简单性和蛋白酶 K 检测的特异性，在生物分析中具有巨大的应用前景。
In this work, kiwi juice functioned as the reducing and capping reagent to obtain stable silver nanoparticles (AgNPs), which was very effective and inexpensive. Importantly, it could be finished within 2 min at room temperature, making the synthesis much faster and easier. Compared with the most common AgNPs (such as sodium citrate coated AgNPs), the proposed AgNPs presented excellent properties such as high stability. Moreover, a new analytical assay was established for protease K with the colour turning from light yellow to brown when protease K was introduced into the AgNPs aqueous solution. It was found that the increase of AgNPs absorbance at 470 nm was proportional to the concentration of protease K in the range of 0.50-15 mg mL(-1) with the limit of detection as low as 0.47 mg mL(-1). This selective and colorimetric assay has a great application prospect in bioanalysis due to its simplicity and specificity for protease K detection.