The neuropeptides and protein hormones of the agricultural pest fruit fly Bactrocera dorsalis: What do we learn from the genome sequencing and tissue-specific transcriptomes?
The neuropeptides and protein hormones of the agricultural pest fruit fly Bactrocera dorsalis: What do we learn from the genome sequencing and tissue-specific transcriptomes?
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农业害虫果蝇橘小实蝇的神经肽和蛋白质激素：我们从基因组测序和组织特异性转录组中学到了什么？
DOI：
10.1016/j.peptides.2017.10.009
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发表时间：
2017-12-01
期刊：
影响因子：
3
通讯作者：
Wang, Jin-Jun
中科院分区：
文献类型：
--
作者：
Gui, Shun-Hua;Jiang, Hong-Bo;Wang, Jin-Jun
神经肽和蛋白质激素是非常重要的信号分子，参与无脊椎动物和脊椎动物各种生理过程的调节和协调。利用生物信息学的方法，对热带和亚热带水果的重要害虫之一桔小实蝇(Bactrocera Dorsalis)的基因组和6个组织特异性转录组数据库(中枢神经系统、脂肪体、卵巢、睾丸、雄性附腺、触角)进行了筛选。39个候选基因被发现编码神经肽或蛋白质激素。这些包括大多数已知的昆虫神经肽和蛋白质激素，除了脂肪动力激素-Corazin相关肽、别拉托品、利尿激素34、利尿激素45、亚胺、肌醇和性肽。我们的结果表明，与其他一些昆虫相比，双翅目昆虫的神经肽和蛋白质激素似乎有一个较少的谱系。此外，桔小蜂与超模黑腹果蝇也有不同之处。桔小实蝇的有趣特征是缺乏编码性肽的基因，存在神经解析素和两个编码神经肽F的基因。已发现的大部分神经肽和蛋白质激素存在于中枢神经系统，只有少数存在于其他组织。通过与FlyBase和FlyAtlas的数据比较，对它们的生理功能进行了预测。综上所述，由于已鉴定的多肽数量众多，本研究可为其他模式昆虫和重要害虫的结构、组织分布和生理功能的比较研究提供参考。
Neuropeptides and protein hormones are very important signaling molecules, and are involved in the regulation and coordination of various physiological processes in invertebrates and vertebrates. Using a bioinformatics approach, we screened the recently sequenced genome and six tissue-specific transcriptome databases (central nervous system, fat body, ovary, testes, male accessory glands, antennae) of the oriental fruit fly (Bactrocera dorsalis) that is economically one of the most important pest insects of tropical and subtropical fruit. Thirty-nine candidate genes were found to encode neuropeptides or protein hormones. These include most of the known insect neuropeptides and protein hormones, with the exception of adipokinetic hormone-corazonin-related peptide, allatropin, diuretic hormone 34, diuretic hormone 45, IMFamide, inotocin, and sex peptide. Our results showed the neuropeptides and protein hormones of Diptera insects appear to have a reduced repertoire compared to some other insects. Moreover, there are also differences between B. dorsalis and the super-model of Drosophila melanogaster. Interesting features of the oriental fruit fly are the absence of genes coding for sex peptide and the presence of neuroparsin and two genes coding neuropeptide F. The majority of the identified neuropeptides and protein hormones is present in the central nervous system, with only a limited number of these in the other tissues. Moreover, we predicted their physiological functions via comparing with data of FlyBase and FlyAtlas. Taken together, owing to the large number of identified peptides, this study can be used as a reference about structure, tissue distribution and physiological functions for comparative studies in other model and important pest insects.