Serum antibodies to the HPV16 proteome as biomarkers for head and neck cancer.
Serum antibodies to the HPV16 proteome as biomarkers for head and neck cancer.
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DOI：
10.1038/bjc.2011.171
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发表时间：
2011-06-07
影响因子：
8.8
通讯作者：
Posner, M.
中科院分区：
文献类型：
--
作者：
Anderson, K. S.;Wong, J.;D'Souza, G.;Riemer, A. B.;Lorch, J.;Haddad, R.;Pai, S. I.;Longtine, J.;McClean, M.;LaBaer, J.;Kelsey, K. T.;Posner, M.
人乳头瘤病毒（HPV）16型与口咽癌（OPC）有关。HPV 16 E6和E7癌蛋白的抗体（Abs）已在患者血清中检测到;然而，其他早期HPV衍生蛋白的Abs尚未得到充分研究。HPV 16蛋白质组的抗体定量使用一种新的多重珠测定，使用C-末端GST融合蛋白捕获到Luminex珠。血清得自未治疗的OPC患者（N=40）、HPV 16 + OPC患者的伴侣（N=11）和健康对照（N=50）。已知病毒样衣壳颗粒+Ab的口咽癌患者血清中HPV 16 E1、E2、E4、E6和E7抗体水平升高，L1抗体水平升高，但E5抗体水平未升高。与对照组相比，E1组p21-GST特异性荧光强度中位数比值为50.7 vs 2.1; E4组为14.6 vs 1.3; E6组为11.3 vs 2.4; E7组为43.1 vs 2.6; L1组为10.3 vs 2.6（均P <0.01）。在验证队列中，HPV 16 E1、E2和E7抗体水平与健康对照样本（P <0.02）和OPC患者伴侣（P <0.01）相比显著升高。HPV 16 + OPC患者具有可检测的E1、E2和E7蛋白的Ab，这些蛋白是HPV相关OPC的潜在生物标志物。
Human papillomavirus (HPV) type 16 is associated with oropharyngeal carcinomas (OPC). Antibodies (Abs) to HPV16 E6 and E7 oncoproteins have been detected in patient sera; however, Abs to other early HPV-derived proteins have not been well explored. Antibodies to the HPV16 proteome were quantified using a novel multiplexed bead assay, using C-terminal GST-fusion proteins captured onto Luminex beads. Sera were obtained from untreated patients with OPC (N=40), partners of patients with HPV16+ OPC (N=11), and healthy controls (N=50). Oropharyngeal carcinomas patients with known virus-like capsid particle+ Abs had elevated serum Abs to HPV16 E1, E2, E4, E6, and E7, and L1 antibody levels, but not E5. The ratios of specific median fluorescence intensity to p21-GST compared with controls were E1: 50.7 vs 2.1; E4: 14.6 vs 1.3; E6: 11.3 vs 2.4; E7: 43.1 vs 2.6; and L1: 10.3 vs 2.6 (each P⩽0.01). In a validation cohort, HPV16 E1, E2, and E7 antibody levels were significantly elevated compared with healthy control samples (P⩽0.02) and partners of OPC patients (P⩽0.01). Patients with HPV16+ OPC have detectable Abs to E1, E2, and E7 proteins, which are potential biomarkers for HPV-associated OPC.
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DOI：
10.1128/cdli.12.8.959-969.2005
发表时间：
2005-08-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Dias, D;Van Doren, J;Esser, MT
通讯作者：
Esser, MT
影响因子：
11.5
作者：
Agrawal, Yuri;Koch, Wayne M.;Xiao, Weihong;Westra, William H.;Trivett, Anna L.;Symer, David E.;Gillison, Maura L.
通讯作者：
Gillison, Maura L.
影响因子：
11.5
作者：
Chung, Christine H.;Gillison, Maura L.
通讯作者：
Gillison, Maura L.
影响因子：
4.4
作者：
Anderson, Karen S.;Ramachandran, Niroshan;LaBaer, Joshua
通讯作者：
LaBaer, Joshua
DOI：
10.1056/nejmoa0912217
发表时间：
2010-07-01
期刊：
The New England journal of medicine
影响因子：
--
作者：
Ang KK;Harris J;Wheeler R;Weber R;Rosenthal DI;Nguyen-Tân PF;Westra WH;Chung CH;Jordan RC;Lu C;Kim H;Axelrod R;Silverman CC;Redmond KP;Gillison ML
通讯作者：
Gillison ML