Generation and molecular analyses of two rheumatoid synovial fluid-derived IgG rheumatoid factors.
Generation and molecular analyses of two rheumatoid synovial fluid-derived IgG rheumatoid factors.
复制标题
两种类风湿滑液衍生的 IgG 类风湿因子的生成和分子分析。
DOI：
10.1002/art.1780360709
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发表时间：
1993
影响因子：
--
通讯作者：
Chen,PP
中科院分区：
文献类型：
--
作者：
Lu,EW;Deftos,M;Olee,T;Huang,DF;Soto-Gil,RW;Carson,DA;Chen,PP
目标。目的研究类风湿滑膜液中IgG - RF （rheumatoid factor, IgG - RF）编码的Ig基因。方法：利用类风湿滑膜液B细胞培养分泌IgG - RF的杂交瘤细胞。结果：获得了两个单特异性IgG - rf。序列分析显示，类风湿滑膜中存在一个新的Vλ基因家族（命名为Vλ9）和广泛的体细胞多样化，包括在高变区重复插入18个核苷酸（6个氨基酸残基）。结论：该数据进一步支持了抗原驱动反应在持续产生潜在致病性IgG - rf中的作用。
Objective.To study the Ig genes that encode IgG rheumatoid factor (IgG‐RF) from rheumatoid synovial fluid.Methods.We used rheumatoid synovial fluid B cells to generate IgG‐RF–secreting hybridomas. We then characterized their binding properties and determined their nucleotide sequences.Results.Two monospecific IgG‐RFs were obtained. Sequence analysis of the RFs revealed a new Vλ gene family (designated Vλ9) and extensive somatic diversification, including a duplication‐insertion of 18 nucleotides (6 amino acid residues) into a hypervariable region.Conclusion.The data provide further support for an antigen‐driven response in the sustained production of potentially pathogenic IgG‐RFs in rheumatoid synovium.