Toward an understanding of the protein interaction network of the human liver.
Toward an understanding of the protein interaction network of the human liver.
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了解人类肝脏的蛋白质相互作用网络
DOI：
10.15252/msb.20178107
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发表时间：
2017-12-18
影响因子：
9.9
通讯作者：
Yang X
中科院分区：
文献类型：
--
作者：
Wang J;Huo K;Ma L;Tang L;Li D;Huang X;Yuan Y;Li C;Wang W;Guan W;Chen H;Jin C;Wei J;Zhang W;Yang Y;Liu Q;Zhou Y;Zhang C;Wu Z;Xu W;Zhang Y;Liu T;Yu D;Zhang Y;Chen L;Zhu D;Zhong X;Kang L;Gan X;Yu X;Ma Q;Yan J;Zhou L;Liu Z;Zhu Y;Zhou T;He F;Yang X
(注：除了更正可能造成歧义的印刷或拼写错误外，信件和报告不进行编辑。信函和推荐人报告的原始格式可能不会反映在本汇编中。)再次感谢你提交你的作品给分子系统生物学。我们现在已经收到了三位同意评估你的手稿的审稿人的回信。正如你将从下面的报告中看到的，推荐人发现你的研究主题有潜在的兴趣。然而，它们引起了重大的关切，我恐怕要说，这些关切使它无法以目前的形式发表。总的来说，审稿人认识到这项工作提供了一个与人类肝脏中表达的蛋白质之间相互作用相关的庞大且潜在有用的数据集。尽管如此，他们提出了一系列问题，他们认为在这项工作适合发表之前，需要令人信服地解决这些问题。我在此强调两个特别重要的问题：审稿人对酵母2杂交筛选的假阳性和假阴性的评估有一些重要的技术问题，以及对co-IP和gst亲和实验中使用的对照的关注。尤其是3号评论者提出的观点。似乎需要进一步澄清，在某些情况下还需要额外的实验控制。2. 审稿人认为，诱饵蛋白的最初选择没有得到充分的描述，他们担心这组靶向肝脏表达蛋白所引入的偏见需要得到充分的描述
(Note: With the exception of the correction of typographical or spelling errors that could be a source of ambiguity, letters and reports are not edited. The original formatting of letters and referee reports may not be reflected in this compilation.) Thank you again for submitting your work to Molecular Systems Biology. We have now heard back from the three referees who agreed to evaluate your manuscript. As you will see from the reports below, the referees find the topic of your study of potential interest. They, however, raise substantial concerns, which, I am afraid to say, preclude its publication in its present form. In general, the reviewers recognized that this work provides a large and potentially useful dataset relevant to interactions among proteins expressed in the human liver. Nonetheless, they raise a series of issues which they felt would need to be addressed convincingly before this work would be appropriate for publications. I highlight here two issues of particular importance: 1. The reviewers had some important technical concerns regarding the assessment of false-positives and false-negatives from the yeast 2-hybrid screen, as well as concerns regarding the controls used in the co-IP and GST-affinity experiments. See, in particular, the points raised by reviewer #3. Additional clarification, and in some cases additional experimental controls, appear needed. 2. The reviewers felt that the initial selection of the bait proteins had not been sufficiently described, and they worried that the bias introduced by this targeted set of liver-expressed proteins needed to