Tetrahydrocannabinol and Two of its Metabolites in Whole Blood Using Liquid Chromatography-Tandem Mass Spectrometry
Tetrahydrocannabinol and Two of its Metabolites in Whole Blood Using Liquid Chromatography-Tandem Mass Spectrometry
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DOI：
10.1093/jat/32.8.653
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发表时间：
2008-10-01
影响因子：
2.5
通讯作者：
Moore, Christine
中科院分区：
文献类型：
--
作者：
Coulter, Cynthia;Miller, Elizabeth;Moore, Christine
建立了全血中Δ-9-四氢大麻酚、11-Nor-9-羧基-Δ-9-四氢大麻酚和11-羟基-Δ-9-四氢大麻酚的分析方法。血样中存在的大麻素用固相萃取定量，然后以正电喷雾电离模式进行MS检测。为了确认，监测了两个转变，并确定了一个比率。被报告为阳性的样品被要求同时存在两个转变，量化转变与合格转变的比率在已知校准标准所确定的比率的20%以内。对合格过渡的监测和对其存在与主离子的特定比例的要求有可能限制分析的灵敏度，然而，对最终结果的额外置信度以及法医防御性被认为更重要。THC、THCA、11-OH-THC的最低定量限分别为0.5、5、2 ng/mL。THC、THCA、11-OH-THC的检出限分别为0.5、4、1 ng/mL。全血中5 ng/mLTHC、11-OH-THC和THCA的回收率分别为71.5%、64.5%和61.2%(n=3)。
An analytical procedure for the determination of Δ9-tetrahydrocannabinol (THC), 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THCA), and 11-hydroxy-Δ9-tetrahydrocannabinol (11-OH-THC) in whole blood has been developed and validated using liquid chromatography with tandem mass spectral detection (MS). Cannabinoids present in the blood samples were quantified using solid-phase extraction followed by MS detection in positive electrospray ionization mode. For confirmation, two transitions were monitored and one ratio determined. Samples being reported as positive were required to have both transitions present, the ratio of quantifying transition to qualifying transition being within 20% of that determined from known calibration standards. The monitoring of the qualifying transition and requirement for its presence within a specific ratio to the primary ion has the potential of limiting the sensitivity of the assay, however, the additional confidence in the final result as well as forensic defensibility were considered to be of greater importance. The limit of quantitation was 0.5 ng/mL for THC, 5 ng/mL for THCA, and 2 ng/mL for 11-OH-THC. The limit of detection was 0.5 ng/mL for THC, 4 ng/mL for THCA, and 1 ng/mL for 11-OH-THC. The percentage recovery of the cannabinoids from whole blood at a concentration of 5 ng/mL was 71.5% for THC, 64.5% for 11-OH-THC, and 61.2% for THCA (n= 3).