L-lactic acid production by simultaneous saccharification and fermentation of dilute ethylediamine pre-treated rice straw
L-lactic acid production by simultaneous saccharification and fermentation of dilute ethylediamine pre-treated rice straw
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稀乙二胺预处理稻草同步糖化发酵生产L-乳酸
DOI：
10.1016/j.indcrop.2019.111749
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发表时间：
2019-12-01
影响因子：
5.9
通讯作者：
Qin, Peiyong
中科院分区：
文献类型：
--
作者：
Chen, Hao;Huo, Weizhong;Qin, Peiyong
为高效生产第二代L-乳酸（L-LA），首次采用稀乙二胺（EDA）对稻草（RS）进行预处理，然后在非灭菌条件下进行流加同步糖化发酵（SSF）。具体而言，预处理参数包括EDA浓度和预处理温度对糖和L-LA生产的影响进行了评估。结果表明，在200 ℃下在6%（w/v）EDA中预处理1 h后，在酶水解后，产生42.25 g L-1的总单体糖。通过分批SSF，使用芽孢杆菌凝结剂LA-15-2可以从粗RS中获得最大化的63.5 +/- 3.0 g L-1和296.8 g Kg（-1）的L-LA。进一步进行补料分批SSF，在最终发酵液中获得92.5 g L-1的L-LA浓度。相应地，预处理RS的L-LA生产率和产量分别达到2.01 g L-1 h（-1）和578.1 g Kg（-1）。该研究为L-乳酸的生产提供了一种新的绿色环保方法，成功地避免了木质纤维素预处理阶段大量无机碱的使用。
To effectively produce second-generation L-lactic acid (L-LA), for the first time, rice straw (RS) was pretreated by dilute ethylenediamine (EDA) and followed by fed-batch simultaneous saccharification and fermentation (SSF) under non-sterilized conditions. Specifically, the impact of pretreatment parameters included EDA concentration and pretreatment temperature on sugars and L-LA productions were evaluated. Results indicated that after pretreatment in 6% (w/v) EDA at 200 degrees C for 1 h and following the enzymatic hydrolysis, 42.25 g L-1 of total monomer sugars was generated. By batch SSF, the maximized 63.5 +/- 3.0 g L-1 and 296.8 g Kg(-1) of L-LA can be obtained from the raw RS using Bacillus coagulant LA-15-2. Fed-batch SSF was further conducted and L-LA concentration of 92.5 g L-1 was obtained in the final fermentation broth. Correspondingly, L-LA productivity and yield reached 2.01 g L-1 h(-1) and 578.1 g Kg(-1) of the pretreated RS, respectively. This study provided a novel environmental-friendly method for L-LA production, which successfully avoided the usage of large amount of inorganic alkali in lignocellulose pretreatment stage.