Distribution of Porphyromonas gingivalis fimA genotypes in chronic apical periodontitis associated with symptoms.
Distribution of Porphyromonas gingivalis fimA genotypes in chronic apical periodontitis associated with symptoms.
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DOI：
10.1016/j.joen.2010.08.018
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发表时间：
2010-11
影响因子：
4.2
通讯作者：
Qian Wang;Xuedong Zhou;Q. Zheng;Yao Wang;Lu Tang;Ding-Ming Huang
中科院分区：
文献类型：
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作者：
Qian Wang;Xuedong Zhou;Q. Zheng;Yao Wang;Lu Tang;Ding-Ming Huang
牙龈卟啉单胞菌（Porphyromonas gingivalis，P. gingivalis）是一种引起根管感染的厌氧菌，其菌毛根据核苷酸序列分为I-V型和Ib型6种基因型。越来越多的证据表明，牙龈卟啉单胞菌与牙周病的一些临床症状有显著的相关性。本研究旨在确定牙龈卟啉单胞菌fimA基因型在根尖周炎中的患病率，并探讨牙龈卟啉单胞菌fimA基因型与根尖周炎临床症状的相关性。提取DNA并用基于聚合酶链反应的鉴定分析进行分析。计算比值比、95%置信区间和列联系数，以将fimA特异性基因与临床疾病联系起来。在39.9%的弯曲根管样本中检测到牙龈卟啉单胞菌，并且在44.5%的牙龈卟啉单胞菌阳性的有症状的样本中发现牙龈卟啉单胞菌。在有症状的病例中，II型（69.4%）最常见，其次是IV型（32.7%）。在无症状根尖周炎中，I型的发生率（64.3%）显著高于其他基因型，而II型和Ib型未被鉴定。统计分析显示，II、IV和Ib型fimA的发生与临床体征的风险更大相关结论：本研究的结果加强了牙龈卟啉单胞菌特异性fimA基因型克隆与根尖周炎之间的关联，表明fimA基因型（II、IV和Ib型）与症状性神经根周围疾病的病因有关。
INTRODUCTIONPorphyromonas gingivalis (P. gingivalis) is an anaerobic bacterium involved in root canal infections whose fimbriae are classified into six genotypes (types I-V and Ib) based on nucleotide sequence. Accumulated evidence suggests there is significant association between P. gingivalis and some clinical symptoms of periodontal diseases. The present study aims to determine the prevalence of P. gingivalis fimA genotypes in apical periodontitis and to investigate the correlation between P. gingivalis fimA genotypes and clinical symptoms.METHODSSamples were obtained from 158 infected root canals with apical periodontitis. DNA was extracted and analyzed with a polymerase chain reaction–based identification assay. Odds ratios, 95% confidence intervals, and contingency coefficient were calculated for associating the fimA-specific genes with clinical symptoms.RESULTSP. gingivalis was detected in 39.9% of the inflected root canal samples and was found in 44.5% of P. gingivalis-positive specimens with symptoms. Types II (69.4%) were the most frequent in the symptomatic cases followed by type IV (32.7%). The occurrence of type I (64.3%) was significantly higher than any other genotypes in the asymptomatic apical periodontitis, whereas type II and type Ib were not identified. Statistical analysis revealed that the occurrences of types II, IV, and Ib fimA were associated with greater risk of clinical signs (swelling, sinus tract, or intracanal exudates) than type I.CONCLUSIONSResults from this study reinforce the association between P. gingivalis–specific fimA genotypic clones and apical periodontitis, indicating that fimA genotypes (types II, IV, and Ib) were related to the etiology of symptomatic periradicular diseases.