Blood plasma separation microfluidic chip with gradual filtration
Blood plasma separation microfluidic chip with gradual filtration
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DOI：
10.1016/j.mee.2014.05.032
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发表时间：
2014-10-05
影响因子：
2.3
通讯作者：
Oo, Maung Kyaw Khaing
中科院分区：
文献类型：
--
作者：
Chen, Jingdong;Chen, Di;Oo, Maung Kyaw Khaing
血液是可以用来诊断疾病的最重要的生物材料之一。为了避免血细胞对游离血浆检测的影响，血液检测的第一步是血液分离。我们开发了一种用于血浆逐步过滤的微流控芯片，该芯片由前端细胞捕获结构和后端过滤器组成。提出了两种滤波片：直线滤波片和方波滤波片。在聚二甲基硅氧烷(PDMS)上制备的细胞捕获结构和过滤器包括两个结构层。第一层由柱子组成，以便在第二层和玻璃之间创建小间隙，这使得等离子体能够通过捕获结构流动，同时将细胞困在结构中。第二层是U形结构的阵列。结果表明，等离子体的分离效率随稀释度的增大和柱层间隙高度的减小而提高。采用直线过滤器的芯片，在2um m间隙和稀释倍数10时，分离效率仅为20%，而在1um m高度和稀释倍数50时，分离效率提高到91%。采用方波滤光片，在芯片高度为1微米，稀释倍数为20的条件下，分离效率接近100%。(C)2014爱思唯尔B.V.保留所有权利。
Blood is one of the most crucial biological materials that can be used to diagnose diseases. In order to avoid the effects of blood cells for cell free plasma detection, the first step toward a blood test is the blood separation. We developed a microfluidic chip for blood plasma separation with gradual filtration, which consisted of front-end cell capture structures and back-end filters. Two types of filters were proposed: straight line filters and square wave filters. The cell capture structures and filters, fabricated on PDMS (polydimethylsiloxane), included two structural layers. The first layer consisted of pillars to create small gaps between the second layer and glass, which enabled the flow of the plasma through the capture structures while trapping the cells in the structures. The second layer was an array of U-shaped structures. The results showed that the separation efficiency of plasma enhanced with increased dilution factor and decreased height of the gap created by the pillar layer. The separation efficiency was only 20% under 2 mu m gap and dilution factor 10 and increased to 91% under the gap of 1 mu m height and dilution factor 50 in the chip with the straight line filters. The separation efficiency was close to 100% under the gap of 1 mu m height and dilution factor 20 in the chip with the square wave filters. (C) 2014 Elsevier B.V. All rights reserved.