Quantitation of latent varicella-zoster virus and herpes simplex virus genomes in human trigeminal ganglia
Quantitation of latent varicella-zoster virus and herpes simplex virus genomes in human trigeminal ganglia
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DOI：
10.1128/jvi.73.12.10514-10518.1999
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发表时间：
1999-12-01
影响因子：
5.4
通讯作者：
Straus, SE
中科院分区：
文献类型：
--
作者：
Pevenstein, SR;Williams, RK;Straus, SE
应用实时荧光PCR技术，对15例三叉神经节内潜伏性水痘带状疱疹病毒（VZV）、单纯疱疹病毒1型（HSV-1）和2型（HSV-2）基因组拷贝数进行了定量分析。15个神经节中有8个（538）和1个（7%）为HSV-1或-2糖蛋白G基因PCR阳性，平均值分别为2，902 +/-1，082（平均值的标准误差）或109个基因组/10（5）个细胞。14例中有11例（79%）至15例中有13例（87%）为VZV基因29、31或62的PCR阳性。合并三种VZV基因的结果得到平均258 +/- 38个基因组/10（5）个神经节细胞。潜伏病毒基因组载量的这些水平对病毒在人类感觉神经节中的分布和再活化具有影响。
Using rear-time fluorescence PCR, we quantitated the numbers of copies of latent varicella-zoster virus (VZV) and herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) genomes in 15 human trigeminal ganglia. Eight (538) and 1 (7%) of 15 ganglia were PCR positive for HSV-1 or -2 glycoprotein G genes, with means of 2,902 +/- 1,082 (standard error of the mean) or 109 genomes/10(5) cells, respectively. Eleven of 14 (79%) to 13 of 15 (87%) of the ganglia were PCR positive for VZV gene 29, 31, or 62. Pooling of the results for the three VZV genes yielded a mean of 258 +/- 38 genomes/10(5) ganglion cells. These levels of latent viral genome loads have implications for virus distribution in and reactivation from human sensory ganglia.