Diverse Central Projection Patterns of Retinal Ganglion Cells.
Diverse Central Projection Patterns of Retinal Ganglion Cells.
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DOI：
10.1016/j.celrep.2017.01.075
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发表时间：
2017-02-21
期刊：
影响因子：
8.8
通讯作者：
Harris JA
中科院分区：
文献类型：
--
作者：
Martersteck EM;Hirokawa KE;Evarts M;Bernard A;Duan X;Li Y;Ng L;Oh SW;Ouellette B;Royall JJ;Stoecklin M;Wang Q;Zeng H;Sanes JR;Harris JA
了解小鼠视网膜中超过30种类型的视网膜神经节细胞（RGC）如何各自对大脑中的视觉处理做出贡献，将需要更多标记和操纵特定RGC的工具。我们使用88个转基因驱动系筛选和分析Cre重组酶在视网膜的表达。在许多细胞系中，Cre在多种RGC类型和视网膜细胞类别中表达，但有几种表现出更多的选择性表达。我们使用病毒示踪剂、高通量成像和数据处理管道，全面绘制了26个Cre系中标记的RGC的中心投影。我们确定了超过50个视网膜受体区域，并提出了一个定量的视网膜到大脑的连接图，使跨线的目标特异性的比较。对两个主要中央目标的投射显着相关;投射到外侧膝状体外壳或核心区域的RGC分别投射到上丘内的浅层或深层。上级丘。视网膜图像和投影数据可在www.connectivity. brain-map. org上在线获得。
Understanding how > 30 types of retinal ganglion cells (RGCs) in the mouse retina each contribute to visual processing in the brain will require more tools that label and manipulate specific RGCs. We screened and analyzed retinal expression of Cre recombinase using 88 transgenic driver lines. In many lines, Cre was expressed in multiple RGC types and retinal cell classes, but several exhibited more selective expression. We comprehensively mapped central projections from RGCs labeled in 26 Cre lines using viral tracers, high-throughput imaging, and a data processing pipeline. We identified over 50 retinorecipient regions, and present a quantitative retina-to-brain connectivity map, enabling comparisons of target-specificity across lines. Projections to two major central targets were notably correlated; RGCs projecting to the outer shell or core regions of the lateral geniculate projected to superficial or deep layers within the superior colliculus, respectively. Retinal images and projection data are available online at www.connectivity.brain-map.org.