Morphometric analysis of lipoprotein-like particle accumulation in aging human macular Bruch's membrane
Morphometric analysis of lipoprotein-like particle accumulation in aging human macular Bruch's membrane
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DOI：
10.1167/iovs.07-1196
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发表时间：
2008-06-01
影响因子：
4.4
通讯作者：
Johnson, Mark
中科院分区：
文献类型：
--
作者：
Huang, Jiahn-Dar;Curcio, Christine A.;Johnson, Mark
目的.目的：探讨脂蛋白样颗粒（LLP）随年龄增长在布鲁赫膜（Bruch's membrane，BrM）中的大小和区域分布。采用快速冷冻/深蚀法制备了27-78岁人脑嵴髓细胞的电镜标本。体视学方法被用来分析所得的显微照片，并确定年龄相关的变化，LLP的体积分数和直径分布在不同的位置在BrM. E. TS。LLP所占的体积分数在内胶原层（ICL）和弹性层（EL）中均随年龄单调增加，但在外胶原层（OCL）中不随年龄增加。总LLP相关的脂质在BrM的质量也随着年龄的增长而增加。LLP大小随年龄增长无显著性增加，但随着BrM中LLP体积分数的增加，LLP大小略有增加。颗粒的蓄积模式与LLP的视网膜色素上皮（RPE）来源一致，这解释了为什么一旦EL和ICL充满颗粒，LLP继续在RPE附近蓄积，但在OCL中未发现进一步蓄积。在BrM中发现的LLP相关脂质的量占该组织中测量的累积脂质的很大一部分。
PURPOSE. To determine the size and regional distribution of lipoprotein-like particles (LLPs) that accumulate with age in Bruch's membrane (BrM).METHODS. The quick-freeze/deep-etch method was used to prepare specimens of human BrM (age range, 27-78) for electron microscopic examination. Stereologic methods were used to analyze the resultant micrographs and determine the age-related changes of the LLP volume fraction and diameter distribution in various locations in BrM.RESULTS. The volume fraction occupied by LLPs was found to increase monotonically with age in both the inner collagenous layer (ICL) and elastic layer (EL), but not in the outer collagenous layer (OCL). The mass of total LLP-associated lipids in BrM also increased with age. There was no significant increase in LLP size with age, but there was a modest increase in size with increased volume fraction of LLPs in BrM.CONCLUSIONS. The pattern of accumulation of particles was consistent with a retinal pigment epithelium (RPE) source for the LLPs, which explains why once the EL and ICL were filled with particles, LLPs continued to accumulate near the RPE, but no further accumulation was found in the OCL. The quantity of LLP-associated lipids found in BrM accounts for a large portion of the accumulated lipids measured in this tissue.